TY - JOUR
T1 - Immunization with Wuchereria bancrofti Glutathione-S-transferase Elicits a Mixed Th1/Th2 Type of Protective Immune Response Against Filarial Infection in Mastomys
AU - Andure, Dhananjay
AU - Pote, Kiran
AU - Khatri, Vishal
AU - Amdare, Nitin
AU - Padalkar, Ramchandra
AU - Reddy, Maryada Venkata Rami
N1 - Funding Information:
The financial support by Department of Biotechnology (DBT) through research project (Project Id. BT/PR/4988/INF/22/155/2012) and FIST grant from Department of Science and Technology (Project Id. SR/FST/LSI-470/2010) both from Ministry of Science & Technology, Government of India is gratefully acknowledged.
Funding Information:
This research work was supported by the research funding received from the Department of Biotechnology (Project Id. BT/PR/4988/INF/22/155/2012) and FIST grant from Department of Science and Technology (Project Id. SR/FST/LSI-470/2010) both from Ministry of Science and Technology, Government of India.
Publisher Copyright:
© 2016, Association of Clinical Biochemists of India.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Lymphatic filariasis is a mosquito borne parasitic infection and can severely affect the normal working ability of an individual. Currently there is no vaccine available to prevent this infection and the development of a potential vaccine could effectively support the on-going mass drug administration program by World Health Organization (WHO). Filarial parasites have complex mechanisms to modulate the host immune responses against them. The glutathione-S-transferases (GST) are the important enzymes effectively involved to counteract the oxidative free radicals produced by the host. In the present study, we have shown that the mastomys which are fully permissible rodents for Brugia malayi when immunized with Wuchereria bancrofti recombinant GST (rWbGST) could induce 65.5 % in situ cytotoxicity against B. malayi infective (L3) larvae. There was a balanced Th1/Th2 immune response in the vaccinated animals, characterized by higher levels of WbGST-specific IgG1 and IgG2a antibodies and pronounced IFN-γ, IL-10 and IL-4 cytokines production by the spleen cells.
AB - Lymphatic filariasis is a mosquito borne parasitic infection and can severely affect the normal working ability of an individual. Currently there is no vaccine available to prevent this infection and the development of a potential vaccine could effectively support the on-going mass drug administration program by World Health Organization (WHO). Filarial parasites have complex mechanisms to modulate the host immune responses against them. The glutathione-S-transferases (GST) are the important enzymes effectively involved to counteract the oxidative free radicals produced by the host. In the present study, we have shown that the mastomys which are fully permissible rodents for Brugia malayi when immunized with Wuchereria bancrofti recombinant GST (rWbGST) could induce 65.5 % in situ cytotoxicity against B. malayi infective (L3) larvae. There was a balanced Th1/Th2 immune response in the vaccinated animals, characterized by higher levels of WbGST-specific IgG1 and IgG2a antibodies and pronounced IFN-γ, IL-10 and IL-4 cytokines production by the spleen cells.
KW - Brugia malayi
KW - Glutathione-S-transferases
KW - Nematodes
KW - Vaccine
KW - Wuchereria bancrofti
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U2 - 10.1007/s12291-016-0556-y
DO - 10.1007/s12291-016-0556-y
M3 - Article
AN - SCOPUS:84957669912
SN - 0970-1915
VL - 31
SP - 423
EP - 430
JO - Indian Journal of Clinical Biochemistry
JF - Indian Journal of Clinical Biochemistry
IS - 4
ER -
