Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix

Qin Xu, Chun Mao, Ni Na Liu, Jun Jie Zhu, Jian Shen

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


A water-soluble chitosan derivation O-carboxymethyl chitosan (CMCS) was developed for the efficient immobilization of horseradish peroxidase (HRP) to construct a H2O2 biosensor. Fourier Transform Infrared (FT-IR) spectrophotometer, Atomic Force Microscope (AFM) and electrochemical method were used to characterize the biosensor. The biosensor was used to determine hydrogen peroxide (H2O2) quantitatively in the presence of methylene blue which was used as a mediator to transfer electrons between the electrode and HRP. It offered a linear range of 2.0 × 10-6-3.7 × 10-5 and 4.2 × 10-5-4.57 × 10-4 M, with a detection limit of 5.32 × 10-7 M estimated at a signal-to-noise ration of 3. The apparent Michaelis-Menten constant (KMapp) of the biosensor was 3.544 × 10-5 M.

Original languageEnglish (US)
Pages (from-to)863-870
Number of pages8
JournalReactive and Functional Polymers
Issue number8
StatePublished - Aug 2006
Externally publishedYes


  • Biosensor
  • Carboxymethylate chitosan
  • HRP
  • Immobilization
  • Water-soluble

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Biochemistry
  • Chemical Engineering(all)
  • Polymers and Plastics
  • Materials Chemistry


Dive into the research topics of 'Immobilization of horseradish peroxidase on O-carboxymethylated chitosan/sol-gel matrix'. Together they form a unique fingerprint.

Cite this