Identification of spermatogenesis in a rat sertoli-cell only model using raman spectroscopy: A feasibility study

E. Charles Osterberg, Melissa A. Laudano, Ranjith Ramasamy, Joshua Sterling, Brian D. Robinson, Marc Goldstein, Philip S. Li, Abigail S. Haka, Peter N. Schlegel

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Purpose We determined whether Raman spectroscopy could identify spermatogenesis in a Sertoli-cell only rat model. Materials and Methods A partial Sertoli-cell only model was created using a testicular hypothermia-ischemia technique. Bilateral testis biopsy was performed in 4 rats. Raman spectra were acquired with a probe in 1 mm3 samples of testicular tissue. India ink was used to mark the site of spectral acquisition. Comparative histopathology was applied to verify whether Raman spectra were obtained from Sertoli-cell only tubules or seminiferous tubules with spermatogenesis. Principal component analysis and logistic regression were used to develop a mathematical model to evaluate the predictive accuracy of identifying tubules with spermatogenesis vs Sertoli-cell only tubules. Results Raman peak intensity changes were noted at 1,000 and 1,690 cm-1 for tubules with spermatogenesis and Sertoli-cell only tubules, respectively. When principal components were used to predict whether seminferous tubules were Sertoli-cell only tubules or showed spermatogenesis, sensitivity and specificity were 96% and 100%, respectively. The ROC AUC to predict tubules with spermatogenesis with Raman spectroscopy was 0.98. Conclusions Raman spectroscopy is capable of identifying seminiferous tubules with spermatogenesis in a Sertoli-cell only ex vivo rat model. Future ex vivo studies of human testicular tissue are necessary to confirm whether these findings can be translated to the clinical setting.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalJournal of Urology
Issue number2
StatePublished - Aug 2014
Externally publishedYes


  • Raman
  • Sertoli cell-only syndrome
  • azoospermia
  • spectrum analysis
  • spermatogenesis
  • testis

ASJC Scopus subject areas

  • Urology


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