Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm

Roxana Martinez-Pinna, Priscila Ramos-Mozo, Julio Madrigal-Matute, Luis M. Blanco-Colio, Juan A. Lopez, Enrique Calvo, Emilio Camafeita, Jes S. Lindholt, Olivier Meilhac, Sandrine Delbosc, Jean Baptiste Michel, Melina Vega De Ceniga, Jesus Egido, Jose L. Martin-Ventura

Research output: Contribution to journalArticlepeer-review

71 Scopus citations


Objective- In the search of novel biomarkers of abdominal aortic aneurysm (AAA) progression, proteins released by intraluminal thrombus (ILT) were analyzed by a differential proteomic approach. Methods and Results- Different layers (luminal/abluminal) of the ILT of AAA were incubated, and the proteins released were analyzed by 2-dimensional difference in-gel electrophoresis. Several differentially expressed proteins involved in main AAA pathological mechanisms (proteolysis, oxidative stress, and thrombosis) were identified by mass spectrometry. Among the proteins identified, peroxiredoxin-1 (PRX-1) was more released by the luminal layer compared with the abluminal layer of the ILT, which was further validated by Western blot, ELISA, and immunohistochemistry. We demonstrated increased PRX-1 serum levels in AAA patients compared with healthy subjects and also positive correlation among PRX-1 and AAA diameter, plasmin-antiplasmin, and myeloperoxidase levels. Finally, a prospective study revealed a positive correlation between PRX-1 serum levels and AAA expansion rate. Moreover, the combination of PRX-1 and AAA size had significantly additive value in predicting growth. Conclusion- Several proteins associated with AAA pathogenesis have been identified by a proteomic approach in ILT-conditioned medium. Among them, PRX-1 serum levels are increased in AAA patients and correlate with AAA size and growth rate, suggesting the potential use of PRX-1 as a biomarker for AAA evolution.

Original languageEnglish (US)
Pages (from-to)935-943
Number of pages9
JournalArteriosclerosis, thrombosis, and vascular biology
Issue number4
StatePublished - Apr 2011
Externally publishedYes


  • aneurysms
  • antioxidants
  • proteomics

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


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