Abstract
Lipid domains of the plasma membrane were originally described as a cell matrix insoluble in cold nonionic detergents and enriched in glycosphingolipids. Because of these biochemical properties, these membrane domains were termed detergent-resistant membranes (DRMs) or detergent-insoluble glycolipid-enriched (DIG) membranes. Membrane rafts and caveolae are two types of lipid domains that share these properties, as well as structural/functional dependence on membrane cholesterol. Membrane rafts and caveolae are believed to act as signaling platforms for ligand-activated receptors, thereby contributing to the regulation of receptor function. Here we describe a simple method to assess the association of GPCRs with detergent resistant membranes in native brain tissue and cultured cells.
Original language | English (US) |
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Pages (from-to) | 411-423 |
Number of pages | 13 |
Journal | Methods in Molecular Biology |
Volume | 746 |
DOIs | |
State | Published - 2011 |
Keywords
- Caveolae
- DIG
- DRM
- Density gradients
- Detergent resistant membranes
- Flotation
- GPCR
- Lipid domains
- Membrane rafts
- mGluR
ASJC Scopus subject areas
- Molecular Biology
- Genetics