TY - JOUR
T1 - Identification and characterization of 3 novel genital human papillomaviruses by overlapping polymerase chain reaction
T2 - CandHPV89, candHPV90, and candHPV91
AU - Terai, Masanori
AU - Burk, Robert D.
N1 - Funding Information:
Received 28 December 2001; revised 19 February 2002; electronically published 31 May 2002. Financial support: National Institutes of Health (to R.D.B.). Reprints or correspondence: Dr. Robert D. Burk, Comprehensive Cancer Center, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461 (burk@aecom.yu.edu).
PY - 2002/6/15
Y1 - 2002/6/15
N2 - Three novel human papillomaviruses (HPVs), candHPV89, candHPV90, and candHPV91, that were previously identified from short polymerase chain reaction (PCR) fragments AE6/CP6108, JC9710, and JC9813, respectively, were cloned and characterized from cervicovaginal cells by use of an overlapping PCR method. The complete nucleotide sequences of candHPV89 (8078 bp), candHPV90 (8033 bp), and candHPV91 (7966 bp) were determined by sequence walking. candHPV89 and candHPV91 were closely related to HPV83 and HPV7 and were placed in the HPV genome homology groups A3 and A8, respectively, by phylogenetic analyses. The genome of candHPV90 was most closely related to HPV71, although these HPV genomes do not seem to form a single lineage, because of the disproportionate divergence of the HPV71 L1 region. On the basis of phylogenetic analyses and available clinical data, these 3 novel HPV genomes appear to have a low oncogenic risk and expand the heterogeneity of HPVs detected in the lower genital tract.
AB - Three novel human papillomaviruses (HPVs), candHPV89, candHPV90, and candHPV91, that were previously identified from short polymerase chain reaction (PCR) fragments AE6/CP6108, JC9710, and JC9813, respectively, were cloned and characterized from cervicovaginal cells by use of an overlapping PCR method. The complete nucleotide sequences of candHPV89 (8078 bp), candHPV90 (8033 bp), and candHPV91 (7966 bp) were determined by sequence walking. candHPV89 and candHPV91 were closely related to HPV83 and HPV7 and were placed in the HPV genome homology groups A3 and A8, respectively, by phylogenetic analyses. The genome of candHPV90 was most closely related to HPV71, although these HPV genomes do not seem to form a single lineage, because of the disproportionate divergence of the HPV71 L1 region. On the basis of phylogenetic analyses and available clinical data, these 3 novel HPV genomes appear to have a low oncogenic risk and expand the heterogeneity of HPVs detected in the lower genital tract.
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U2 - 10.1086/340824
DO - 10.1086/340824
M3 - Article
C2 - 12085327
AN - SCOPUS:0037097771
SN - 0022-1899
VL - 185
SP - 1794
EP - 1797
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 12
ER -