Hydroxyl versus permethylated glycopolymers as gene carriers

Juan Alfonso Redondo, Enrique Martínez-Campos, Rodrigo Navarro, Mónica Pérez-Perrino, Helmut Reinecke, Alberto Gallardo, Guillermo Corrales, Alfonso Fernández-Mayoralas, Carlos Elvira

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The main parameters that contribute to non-viral gene delivery are chemical structure and charge distribution. Indeed, saccharide units have been reported to have specific interactions with proteins located in the outer leaflet of the plasma cell membrane that facilitate the cellular internalization of plasmid-DNA vector complexes. In this work, glycopolymers based on statistical copolymers were synthesized through radical copolymerization of a cationic unit, N-ethyl pyrrolidine methacrylamide (EPA), with two styrenic monomers derived from the hydroxylated and permethylated forms of α-glucose. These copolymers were evaluated as possible non-viral gene carriers, and their ability to complex DNA was evaluated. The transfection efficiency and cytocompatibility of the polyplexes, in both fibroblastic and tumoral murine cell lines, was evaluated. Systems derived from α-glucose (GLCSt), over a monomer concentration range of 5–70 mol%, exhibited high toxicity and low transfection efficiency, and were not able to significantly improve on results obtained from positive poly-EPA (PEPA) and polyethyleneimine (PEI) controls. However, systems derived from the permethylated form of α-glucose (MGLCSt), formed stable complexes with DNA or polyplexes, which showed improved transfection efficiency and cytocompatibility in comparison to positive controls. The high transfection efficiency can be clearly attributed to their cytocompatibility, which was notably found to be different for Swiss fibroblasts and B16 melanoma cells, high for Swiss and low for B16. As such, we present permethylated MCLCSt copolymers as good candidates for the possible development of therapies against melanoma.

Original languageEnglish (US)
Pages (from-to)68-76
Number of pages9
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
StatePublished - Aug 1 2017
Externally publishedYes


  • Gene therapy
  • Glucose
  • Polymers
  • Transfection

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science


Dive into the research topics of 'Hydroxyl versus permethylated glycopolymers as gene carriers'. Together they form a unique fingerprint.

Cite this