Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry

Janna G. Kiselar; Steven C. Almo; Paul A. Janmey; Mark R. Chance

Hydroxyl radical probe of Ca ²⁺ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry

Janna G. Kiselar, Steven C. Almo, Paul A. Janmey, Mark R. Chance

Biochemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

The Ca ²⁺ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca ²⁺ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.

Original language	English (US)
Pages	363-364
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Hydroxyl radical probe of Ca ²⁺ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry. / Kiselar, Janna G.; Almo, Steven C.; Janmey, Paul A. et al.
2002. 363-364 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

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title = "Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry",

abstract = "The Ca 2+ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca 2+ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.",

author = "Kiselar, {Janna G.} and Almo, {Steven C.} and Janmey, {Paul A.} and Chance, {Mark R.}",

year = "2002",

language = "English (US)",

pages = "363--364",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - CONF

T1 - Hydroxyl radical probe of Ca 2+ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry

AU - Kiselar, Janna G.

AU - Almo, Steven C.

AU - Janmey, Paul A.

AU - Chance, Mark R.

PY - 2002

Y1 - 2002

N2 - The Ca 2+ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca 2+ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.

AB - The Ca 2+ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca 2+ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442661988&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442661988

SP - 363

EP - 364

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Hydroxyl radical probe of Ca ²⁺ activated gelsolin structure using synchrotron X-ray radiolysis and mass spectrometry

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ASJC Scopus subject areas

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