The Ca 2+ activated gelsolin structure was investigated for hydroxyl radical using synchrotron x-ray radiolysis and mass spectrometry. Inactivated and activated forms of gelsolin were exposed to a synchrotron light for 20-160 millisecond, and irradiated protein solutions were subjected to proteolysis. Dose response profiles for oxidized peptides in the absence and presence of Ca 2+ were constructed. Hydroxyl radicals, produced by radiolysis with synchrotron radiation, resulted in the efficient oxidation of aromatic and sulfur-containing amino acids of gelsolin protein in proportion to their solvent accessibility.
|Number of pages
|Published - 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002 → Jun 6 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
|6/2/02 → 6/6/02
ASJC Scopus subject areas