Human type II collagen gene (COL2A1) assigned to chromosome 12q13.1-q13.2 by in situ hybridization with biotinylated DNA probe

Ei ichi Takahashi; Tada aki Hori; Jeanne B. Lawrence; John McNeil; Robert H. Singer; Peter O'Connell; Mark Leppert; Ray White

doi:10.1007/BF01929213

Human type II collagen gene (COL2A1) assigned to chromosome 12q13.1-q13.2 by in situ hybridization with biotinylated DNA probe

Ei ichi Takahashi, Tada aki Hori, Jeanne B. Lawrence, John McNeil, Robert H. Singer, Peter O'Connell, Mark Leppert, Ray White

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45 Scopus citations

Abstract

We have made a regional assignment of the type II collagen gene (COL2A1) on human chromosome 12 by means of an in situ hybridization technique with a biotinylated DNA probe. The precise localization of the signal was mapped to the band 12q13.1-q13.2. This result was in agreement with the previous mapping by isotopic in situ hybridization technique (12q13.1-q13.2), but not with the result of Southern hybridization analysis using somatic cell hybrids (12q14.3).

Original language	English (US)
Pages (from-to)	307-311
Number of pages	5
Journal	The Japanese Journal of Human Genetics
Volume	34
Issue number	4
DOIs	https://doi.org/10.1007/BF01929213
State	Published - Dec 1989
Externally published	Yes

Keywords

COL2A1 and chromosome 12
chromosome mapping
nonisotopic in situ hybridization

ASJC Scopus subject areas

Genetics(clinical)

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10.1007/BF01929213

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title = "Human type II collagen gene (COL2A1) assigned to chromosome 12q13.1-q13.2 by in situ hybridization with biotinylated DNA probe",

abstract = "We have made a regional assignment of the type II collagen gene (COL2A1) on human chromosome 12 by means of an in situ hybridization technique with a biotinylated DNA probe. The precise localization of the signal was mapped to the band 12q13.1-q13.2. This result was in agreement with the previous mapping by isotopic in situ hybridization technique (12q13.1-q13.2), but not with the result of Southern hybridization analysis using somatic cell hybrids (12q14.3).",

keywords = "COL2A1 and chromosome 12, chromosome mapping, nonisotopic in situ hybridization",

author = "Takahashi, {Ei ichi} and Hori, {Tada aki} and Lawrence, {Jeanne B.} and John McNeil and Singer, {Robert H.} and Peter O'Connell and Mark Leppert and Ray White",

year = "1989",

month = dec,

doi = "10.1007/BF01929213",

language = "English (US)",

volume = "34",

pages = "307--311",

journal = "The Japanese Journal of Human Genetics",

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TY - JOUR

T1 - Human type II collagen gene (COL2A1) assigned to chromosome 12q13.1-q13.2 by in situ hybridization with biotinylated DNA probe

AU - Takahashi, Ei ichi

AU - Hori, Tada aki

AU - Lawrence, Jeanne B.

AU - McNeil, John

AU - Singer, Robert H.

AU - O'Connell, Peter

AU - Leppert, Mark

AU - White, Ray

PY - 1989/12

Y1 - 1989/12

N2 - We have made a regional assignment of the type II collagen gene (COL2A1) on human chromosome 12 by means of an in situ hybridization technique with a biotinylated DNA probe. The precise localization of the signal was mapped to the band 12q13.1-q13.2. This result was in agreement with the previous mapping by isotopic in situ hybridization technique (12q13.1-q13.2), but not with the result of Southern hybridization analysis using somatic cell hybrids (12q14.3).

AB - We have made a regional assignment of the type II collagen gene (COL2A1) on human chromosome 12 by means of an in situ hybridization technique with a biotinylated DNA probe. The precise localization of the signal was mapped to the band 12q13.1-q13.2. This result was in agreement with the previous mapping by isotopic in situ hybridization technique (12q13.1-q13.2), but not with the result of Southern hybridization analysis using somatic cell hybrids (12q14.3).

KW - COL2A1 and chromosome 12

KW - chromosome mapping

KW - nonisotopic in situ hybridization

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ER -

Human type II collagen gene (COL2A1) assigned to chromosome 12q13.1-q13.2 by in situ hybridization with biotinylated DNA probe

Abstract

Keywords

ASJC Scopus subject areas

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