Human SMARCA5 is continuously required to maintain nucleosome spacing

Monica L. Bomber, Jing Wang, Qi Liu, Kelly R. Barnett, Hillary M. Layden, Emily Hodges, Kristy R. Stengel, Scott W. Hiebert

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Genetic models suggested that SMARCA5 was required for DNA-templated events including transcription, DNA replication, and DNA repair. We engineered a degron tag into the endogenous alleles of SMARCA5, a catalytic component of the imitation switch complexes in three different human cell lines to define the effects of rapid degradation of this key regulator. Degradation of SMARCA5 was associated with a rapid increase in global nucleosome repeat length, which may allow greater chromatin compaction. However, there were few changes in nascent transcription within the first 6 h of degradation. Nevertheless, we demonstrated a requirement for SMARCA5 to control nucleosome repeat length at G1/S and during the S phase. SMARCA5 co-localized with CTCF and H2A.Z, and we found a rapid loss of CTCF DNA binding and disruption of nucleosomal phasing around CTCF binding sites. This spatiotemporal analysis indicates that SMARCA5 is continuously required for maintaining nucleosomal spacing.

Original languageEnglish (US)
Pages (from-to)507-522.e6
JournalMolecular Cell
Issue number4
StatePublished - Feb 16 2023


  • ATAC-seq
  • CTCF
  • H2A.Z
  • MNase-seq
  • PRO-seq
  • chromatin
  • nucleosome repeat length

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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