@article{d0c34230d68d4c838d2ab9b73ad7b58e,
title = "Human papillomaviruses of different types in precancerous lesions of the uterine cervix. Histologic, immunocytochemical and ultrastructural studies",
abstract = "In a prospective study of 34 women with abnormal Papanicolaou smears, biopsy and cervicovaginal lavage specimens were analyzed for the presence of human papillomaviruses (HPVs) by Southern blot analysis and probes for HPVs 6, 11, 16, and 18. In 22 of the 23 patients with cervical lesions (96%), HPV DNA was identified in one or more specimens. All patients in whom HPV DNA was found had either koilocytotic or dysplastic lesions on biopsy or Papanicolaou smear. Immunocytochemical demonstration of HPV in biopsy samples was associated with the presence of large amounts of HPV DNA and with the ultrastructural identification of viral particles. The presence of HPV DNA in cervical biopsy specimens was limited to discrete geographic areas of the cervix with histologic abnormalities. Although HPV 16 and other related HPV types were found in all cases of severe cervical intraepithelial neoplasia, the type of HPV present in a given specimen could not be predicted on the basis of morphologic, immunocytochemical, or electron microscopic findings. It is concluded that virtually all dysplastic lesions of the cervix contain HPV DNA, that HPV is thus likely to be a major etiologic agent in the pathogenesis of cervical dysplasia, and that histopathologic features are not predictive of HPV type.",
author = "Annas Kadish and Burk, {Robert D.} and Yvonne Kress and Servio Calderin and Romney, {Seymour L.}",
note = "Funding Information: Epidemiologic evidence supports a role for an infections agent in tile I)atllogenesis of cervical cancer{"} Several viral and nonviral agents, inchtding herpes simplex virus, l Chlamydia trachomatis,'-' and pa-pillonmvirttses, :~.t have' been sttggested as possible etiologic agents in this disease. Morphologic evidence for the involvement of llumatl l)al)illomaviruses (HPVs) in precancerons lesions of tile cervix itlcludes the presence of koilocytotic atypia and warty cilanges in cervical biopsy specimens and cytologic smears with dysplastic changes, the demotlstration of viral antigens in these tissnes by itnmtmocytochenlical techtfiques, 5,6 and the identification of viral particles by electron microscopy. 7 With DNA hybridization teclmiques, DNA ft-om HPV types 6 and 1 1 has been identified in condylomata accunlinata of the genital tract, in fiat condylomata of tile cervix, and in laryngeal papillotnas, but it has been reported only rarely in carcinotnas, s Recently, DNA fl'om two new HPV types, types 16 and 18, was found in cervical cancers, Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York. Revision accepted for lmblicatiou September 6, 1985. Supported by Nltl grants CA29906 and CA13330; American Cancer Society grant IN-28-7; a grant fl-om the Brookdale Foundation; and a clinical investigator award (KO8-CA-00983) from the National Cancer Institute, DtttlS (R.D.B.). Address correspondence and reprint requests to Dr. Kadish: Department of l'athology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461.",
year = "1986",
month = apr,
doi = "10.1016/S0046-8177(86)80462-2",
language = "English (US)",
volume = "17",
pages = "384--392",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "4",
}