HR22C16: A potent small-molecule probe for the dynamics of cell division

Srinivas Hotha; Justin C. Yarrow; Janet G. Yang; Sarah Garrett; Kishore V. Renduchintala; Thomas U. Mayer; Tarun M. Kapoor

doi:10.1002/anie.200351173

HR22C16: A potent small-molecule probe for the dynamics of cell division

Srinivas Hotha, Justin C. Yarrow, Janet G. Yang, Sarah Garrett, Kishore V. Renduchintala, Thomas U. Mayer, Tarun M. Kapoor

Research output: Contribution to journal › Article › peer-review

141 Scopus citations

Abstract

A high-throughput, microscopy-based chemical-genetic screen identified HR22C16, which causes a monoastral mitotic block, as a small-molecule probe for cell division (see picture). By using a diastereoselective, traceless solid-phase synthesis and biological assays, a more potent HR22C16 analogue was then identified. A photocaging strategy for HR22C16 was also developed to allow fast temporal control over the function of the target protein Eg5.

Original language	English (US)
Pages (from-to)	2379-2382
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	42
Issue number	21
DOIs	https://doi.org/10.1002/anie.200351173
State	Published - May 30 2003
Externally published	Yes

Keywords

Antimitotics
Inhibitors
Molecular motors
Photolysis
Solid-phase synthesis

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200351173

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abstract = "A high-throughput, microscopy-based chemical-genetic screen identified HR22C16, which causes a monoastral mitotic block, as a small-molecule probe for cell division (see picture). By using a diastereoselective, traceless solid-phase synthesis and biological assays, a more potent HR22C16 analogue was then identified. A photocaging strategy for HR22C16 was also developed to allow fast temporal control over the function of the target protein Eg5.",

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AU - Yarrow, Justin C.

AU - Yang, Janet G.

AU - Garrett, Sarah

AU - Renduchintala, Kishore V.

AU - Mayer, Thomas U.

AU - Kapoor, Tarun M.

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AB - A high-throughput, microscopy-based chemical-genetic screen identified HR22C16, which causes a monoastral mitotic block, as a small-molecule probe for cell division (see picture). By using a diastereoselective, traceless solid-phase synthesis and biological assays, a more potent HR22C16 analogue was then identified. A photocaging strategy for HR22C16 was also developed to allow fast temporal control over the function of the target protein Eg5.

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KW - Inhibitors

KW - Molecular motors

KW - Photolysis

KW - Solid-phase synthesis

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HR22C16: A potent small-molecule probe for the dynamics of cell division

Abstract

Keywords

ASJC Scopus subject areas

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