HR22C16: A potent small-molecule probe for the dynamics of cell division

Srinivas Hotha, Justin C. Yarrow, Janet G. Yang, Sarah Garrett, Kishore V. Renduchintala, Thomas U. Mayer, Tarun M. Kapoor

Research output: Contribution to journalArticlepeer-review

142 Scopus citations


A high-throughput, microscopy-based chemical-genetic screen identified HR22C16, which causes a monoastral mitotic block, as a small-molecule probe for cell division (see picture). By using a diastereoselective, traceless solid-phase synthesis and biological assays, a more potent HR22C16 analogue was then identified. A photocaging strategy for HR22C16 was also developed to allow fast temporal control over the function of the target protein Eg5.

Original languageEnglish (US)
Pages (from-to)2379-2382
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number21
StatePublished - May 30 2003
Externally publishedYes


  • Antimitotics
  • Inhibitors
  • Molecular motors
  • Photolysis
  • Solid-phase synthesis

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry


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