Histochemical characteristics of the striated inclusions of adrenoleukodystrophy

A. B. Johnson, H. H. Schaumburg, J. M. Powers

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


The striated accumulations in adrenal cortical cells and brain macrophages that are characteristic of adrenoleukodystrophy were studied histochemically in cryostat sections to seek leads for the biochemical identification of the striated material. It stained pale pink with oil red O and did not stain with the Schultz cholesterol procedure or periodic acid Schiff technique. By utilizing the birefringence of the accumulations as a marker, it was determined that, unlike natural cholesterol and cholesterol esters, the striated material was resistant to acetone and ethanol extraction. It was readily soluble, however, in nonpolar solvents such as n hexane and chloroform. These findings indicated that the material was most probably a lipid, and suggested that sequential extraction of adrenoleukodystrophy adrenal and brain with acetone and then n hexane could be used to isolate this material in relatively pure form. Based on this lead, biochemical studies have just revealed a fatty acid abnormality in adrenoleukodystrophy which appears to be unique to this genetic disease.

Original languageEnglish (US)
Pages (from-to)725-730
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Issue number6
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Anatomy
  • Histology


Dive into the research topics of 'Histochemical characteristics of the striated inclusions of adrenoleukodystrophy'. Together they form a unique fingerprint.

Cite this