TY - JOUR
T1 - Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization
AU - Lawrence, Jeanne Bentley
AU - Singer, Robert H.
AU - Marselle, Lisa M.
N1 - Funding Information:
We would especially like to acknowledge the excellent technlcal contribution of Carol Villnave Johnson during the early phase of this work. We thank Marie Georgio for her expertise in color photographic processing and Adam Singer for his assistance with this. We appreciate the technical assistance of John McNeil, the administrative assistance of Elayn Byron, and the gift of the neu oncogene-transfected 3T3 cells from Applied Biotechnology. We thank Ted Fey for thoughtful reading of the manuscript. This work was supported by grant HD18066 from the National Institute of Health to R. H. S., and J. B. L. and a Muscular Dystrophy Association Grant to J. B. L.
PY - 1989/5/5
Y1 - 1989/5/5
N2 - Use of in situ hybridization optimized for fluorescent detection of nuclear RNA has revealed a striking localization of specific viral RNAs within nuclei of cells latently infected with EBV. Several hundred kb of specific transcripts is sharply restricted to a small region of the nucleus, frequently in a curvilinear "track." Detection of nuclear RNA was evidenced by hybridization without denaturation, sensitivity to RNAase, inhibition by actinomycin D, and specificity of transcribed sequences. Results indicate that RNA "tracks" extend from an internal genome into the nuclear periphery, and that RNA transport may be coupled to transcription. Localized nRNA is apparent for other viral sequences, different lymphoblastoid cell lines, nuclei prepared by two different methods, and an abundant, nonviral transfected sequence. Implications for understanding nuclear organization and the investigation of gene expression are discussed.
AB - Use of in situ hybridization optimized for fluorescent detection of nuclear RNA has revealed a striking localization of specific viral RNAs within nuclei of cells latently infected with EBV. Several hundred kb of specific transcripts is sharply restricted to a small region of the nucleus, frequently in a curvilinear "track." Detection of nuclear RNA was evidenced by hybridization without denaturation, sensitivity to RNAase, inhibition by actinomycin D, and specificity of transcribed sequences. Results indicate that RNA "tracks" extend from an internal genome into the nuclear periphery, and that RNA transport may be coupled to transcription. Localized nRNA is apparent for other viral sequences, different lymphoblastoid cell lines, nuclei prepared by two different methods, and an abundant, nonviral transfected sequence. Implications for understanding nuclear organization and the investigation of gene expression are discussed.
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U2 - 10.1016/0092-8674(89)90924-0
DO - 10.1016/0092-8674(89)90924-0
M3 - Article
C2 - 2541917
AN - SCOPUS:0024498788
SN - 0092-8674
VL - 57
SP - 493
EP - 502
JO - Cell
JF - Cell
IS - 3
ER -