To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.
|Original language||English (US)|
|Number of pages||7|
|Journal||Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis|
|State||Published - Jan 1 1991|
- LacZ shuttle vector
- Mouse X-chromosome
- Mutational 'hot spot'
- Spontaneous mutations
- Transgenic mice
ASJC Scopus subject areas
- Molecular Biology
- Health, Toxicology and Mutagenesis