High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome

Jan A. Gossen; Wiljo J.F. de Leeuw; Aart Verwest; Paul H.M. Lohman; Jan Vijg

doi:10.1016/0027-5107(91)90198-W

High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome

Jan A. Gossen, Wiljo J.F. de Leeuw, Aart Verwest, Paul H.M. Lohman, Jan Vijg

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of ^-5 - 10^-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.

Original language	English (US)
Pages (from-to)	423-429
Number of pages	7
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	250
Issue number	1-2
DOIs	https://doi.org/10.1016/0027-5107(91)90198-W
State	Published - 1991
Externally published	Yes

Keywords

LacZ shuttle vector
Mouse X-chromosome
Mutational 'hot spot'
Spontaneous mutations
Transgenic mice

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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10.1016/0027-5107(91)90198-W

Cite this

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title = "High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome",

abstract = "To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.",

keywords = "LacZ shuttle vector, Mouse X-chromosome, Mutational 'hot spot', Spontaneous mutations, Transgenic mice",

author = "Gossen, {Jan A.} and {de Leeuw}, {Wiljo J.F.} and Aart Verwest and Lohman, {Paul H.M.} and Jan Vijg",

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doi = "10.1016/0027-5107(91)90198-W",

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T1 - High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome

AU - Gossen, Jan A.

AU - de Leeuw, Wiljo J.F.

AU - Verwest, Aart

AU - Lohman, Paul H.M.

AU - Vijg, Jan

PY - 1991

Y1 - 1991

N2 - To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.

AB - To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.

KW - LacZ shuttle vector

KW - Mouse X-chromosome

KW - Mutational 'hot spot'

KW - Spontaneous mutations

KW - Transgenic mice

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High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome

Abstract

Keywords

ASJC Scopus subject areas

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