High-resolution multiphoton imaging of tumors in vivo

Jeffrey Wyckoff, Bojana Gligorijevic, David Entenberg, Jeffrey Segall, John Condeelis

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Analysis of the individual steps in metastasis is crucial if insights at the molecular level are to be linked to the cell biology of cancer. A technical hurdle to achieving the analysis of the individual steps of metastasis is the fact that, at the gross level, tumors are heterogeneous in both animal models and patients. Human primary tumors show extensive variation in all properties ranging from growth and morphology of the tumor through tumor-cell density in the blood and formation and growth of metastases. Methods capable of the direct visualization and analysis of tumor-cell behavior at single-cell resolution in vivo have become crucial in advancing the understanding of mechanisms of metastasis, the definition of microenvironment, and the markers related to both. This article discusses the use of high-resolution multiphoton imaging of tumors (specifically breast tumors in mice) in vivo.

Original languageEnglish (US)
Pages (from-to)1167-1184
Number of pages18
JournalCold Spring Harbor Protocols
Issue number10
StatePublished - Oct 2011
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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