Herpes simplex virus 1 induces de novo phospholipid synthesis

Esther Sutter, Anna Paula de Oliveira, Kurt Tobler, Elisabeth M. Schraner, Sabrina Sonda, Andres Kaech, Miriam S. Lucas, Mathias Ackermann, Peter Wild

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Herpes simplex virus type 1 capsids bud at nuclear membranes and Golgi membranes acquiring an envelope composed of phospholipids. Hence, we measured incorporation of phospholipid precursors into these membranes, and quantified changes in size of cellular compartments by morphometric analysis. Incorporation of [ 3H]-choline into both nuclear and cytoplasmic membranes was significantly enhanced upon infection. [ 3H]-choline was also part of isolated virions even grown in the presence of brefeldin A. Nuclei expanded early in infection. The Golgi complex and vacuoles increased substantially whereas the endoplasmic reticulum enlarged only temporarily. The data suggest that HSV-1 stimulates phospholipid synthesis, and that de novo synthesized phospholipids are inserted into nuclear and cytoplasmic membranes to i) maintain membrane integrity in the course of nuclear and cellular expansion, ii) to supply membrane constituents for envelopment of capsids by budding at nuclear membranes and Golgi membranes, and iii) to provide membranes for formation of transport vacuoles.

Original languageEnglish (US)
Pages (from-to)124-135
Number of pages12
Issue number2
StatePublished - Aug 1 2012
Externally publishedYes


  • Golgi membranes
  • Herpes virus
  • Intracellular traffic
  • Morphometry
  • Nuclear membranes
  • Phospholipid synthesis
  • SEM
  • TEM

ASJC Scopus subject areas

  • Virology


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