TY - JOUR
T1 - Hemoglobin miyada
T2 - DNA analysis of the anti‐lepore βδ fusion gene
AU - Driscoll, M. Catherine
AU - Ohta, Yoshiro
AU - Nakamura, Frank
AU - Bloom, Arthur
AU - Bank, Arthur
PY - 1984/12
Y1 - 1984/12
N2 - Hemoglobin Miyada, an anti‐Lepore hemoglobin, represents the protein product of a nonhomologous crossover between β and δ genes. The mutant globin is β‐like from the N terminus to amino acid 12, and δ‐like from amino acid 22 through the C terminus, thus predicting a crossover site in the first coding region of the gene. DNA analysis, using multiple restriction endonucleases and hybridization to δ and β globin gene‐specific probes, confirms that the βδ hybrid gene 1) is located on a single chromosomal fragment between normal δ and β genes, and 2) has 5′ β promoter sequences, δ IVS 1 and 2, and 3′ coding and flanking sequences.
AB - Hemoglobin Miyada, an anti‐Lepore hemoglobin, represents the protein product of a nonhomologous crossover between β and δ genes. The mutant globin is β‐like from the N terminus to amino acid 12, and δ‐like from amino acid 22 through the C terminus, thus predicting a crossover site in the first coding region of the gene. DNA analysis, using multiple restriction endonucleases and hybridization to δ and β globin gene‐specific probes, confirms that the βδ hybrid gene 1) is located on a single chromosomal fragment between normal δ and β genes, and 2) has 5′ β promoter sequences, δ IVS 1 and 2, and 3′ coding and flanking sequences.
KW - gene analysis
KW - hemoglobin Miyada
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U2 - 10.1002/ajh.2830170405
DO - 10.1002/ajh.2830170405
M3 - Article
C2 - 6093505
AN - SCOPUS:0021709805
SN - 0361-8609
VL - 17
SP - 355
EP - 362
JO - American Journal of Hematology
JF - American Journal of Hematology
IS - 4
ER -