@inbook{8d9a32ce809247d5ab0f799777c69e18,
title = "Genome wide mapping of methylated and hydroxyl-methylated cytosines using a modified hpaii tiny fragment enrichment by ligation mediated PCR tagged sequencing protocol",
abstract = "Here we describe a method for genome wide investigation of methylation and hydroxymethylation status of cytosines. This protocol is an improvement of the HELP-tagging protocol previously described by Suzuki et al. It involves the glucosylation of 5-hydroxymethylcytosines (5-hmC) with β-glucosyl transferase (β-GT), thus rendering them resistant to digestion by MspI. Parallel digestion of β-GT treated samples with MspI, untreated sample with MspI and another untreated sample with HpaII, followed by adapter ligation, parallel sequencing and bioinformatics processing results in a differential display of MspI digestion sites that allows the determination of the distribution of 5-methylcytosines (5-mC) and 5-hmC at these sites.",
keywords = "5-hydroxymethylcytosine, 5-methylcytosine, DNA methylation, HpaII, MspI, Sequencing, β-glucosyl transferase",
author = "Sanchari Battachariyya and Ruslana Tytarenko and Christoph Heuck and John Greally and Amit Verma",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7865-6_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "167--177",
booktitle = "Methods in Molecular Biology",
}