Genome-wide association and functional studies identify a role for IGFBP3 in hip osteoarthritis

Daniel S. Evans, Frederic Cailotto, Neeta Parimi, Ana M. Valdes, Martha C. Castaño-Betancourt, Youfang Liu, Robert C. Kaplan, Martin Bidlingmaier, Ramachandran S. Vasan, Alexander Teumer, Gregory J. Tranah, Michael C. Nevitt, Steven R. Cummings, Eric S. Orwoll, Elizabeth Barrett-Connor, Jordan B. Renner, Joanne M. Jordan, Michael Doherty, Sally A. Doherty, Andre G. UitterlindenJoyce B.J. Van Meurs, Tim D. Spector, Rik J. Lories, Nancy E. Lane

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Objectives: To identify genetic associations with hip osteoarthritis (HOA), we performed a meta-analysis of genome-wide association studies (GWAS) of HOA. Methods: The GWAS meta-analysis included approximately 2.5 million imputed HapMap single nucleotide polymorphisms (SNPs). HOA cases and controls defined radiographically and by total hip replacement were selected from the Osteoporotic Fractures in Men (MrOS) Study and the Study of Osteoporotic Fractures (SOF) (654 cases and 4697 controls, combined). Replication of genome-wide significant SNP associations (p ≤5×10-8) was examined in five studies (3243 cases and 6891 controls, combined). Functional studies were performed using in vitro models of chondrogenesis and osteogenesis. Results: The A allele of rs788748, located 65 kb upstream of the IGFBP3 gene, was associated with lower HOA odds at the genome-wide significance level in the discovery stage (OR 0.71, p=2×10-8). The association replicated in five studies (OR 0.92, p=0.020), but the joint analysis of discovery and replication results was not genome-wide significant (p=1×10-6). In separate study populations, the rs788748 A allele was also associated with lower circulating IGFBP3 protein levels (p=4×10-13), suggesting that this SNP or a variant in linkage disequilibrium could be an IGFBP3 regulatory variant. Results from functional studies were consistent with association results. Chondrocyte hypertrophy, a deleterious event in OA pathogenesis, was largely prevented upon IGFBP3 knockdown in chondrocytes. Furthermore, IGFBP3 overexpression induced cartilage catabolism and osteogenic differentiation. Conclusions: Results from GWAS and functional studies provided suggestive links between IGFBP3 and HOA.

Original languageEnglish (US)
Pages (from-to)1861-1867
Number of pages7
JournalAnnals of the Rheumatic Diseases
Issue number10
StatePublished - Oct 1 2015

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology
  • General Biochemistry, Genetics and Molecular Biology


Dive into the research topics of 'Genome-wide association and functional studies identify a role for IGFBP3 in hip osteoarthritis'. Together they form a unique fingerprint.

Cite this