Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes

Ryan C. Ransom; Deshka S. Foster; Ankit Salhotra; Ruth Ellen Jones; Clement D. Marshall; Tripp Leavitt; Matthew P. Murphy; Alessandra L. Moore; Charles P. Blackshear; Derrick C. Wan; Michael T. Longaker

doi:10.1038/s41467-018-05436-6

Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes

Ryan C. Ransom, Deshka S. Foster, Ankit Salhotra, Ruth Ellen Jones, Clement D. Marshall, Tripp Leavitt, Matthew P. Murphy, Alessandra L. Moore, Charles P. Blackshear, Derrick C. Wan, Michael T. Longaker

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreER^T2 recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.

Original language	English (US)
Article number	2971
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-05436-6
State	Published - Dec 1 2018
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41467-018-05436-6

Cite this

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title = "Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes",

abstract = "Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreERT2 recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.",

author = "Ransom, {Ryan C.} and Foster, {Deshka S.} and Ankit Salhotra and Jones, {Ruth Ellen} and Marshall, {Clement D.} and Tripp Leavitt and Murphy, {Matthew P.} and Moore, {Alessandra L.} and Blackshear, {Charles P.} and Wan, {Derrick C.} and Longaker, {Michael T.}",

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doi = "10.1038/s41467-018-05436-6",

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AU - Ransom, Ryan C.

AU - Foster, Deshka S.

AU - Salhotra, Ankit

AU - Jones, Ruth Ellen

AU - Marshall, Clement D.

AU - Leavitt, Tripp

AU - Murphy, Matthew P.

AU - Moore, Alessandra L.

AU - Blackshear, Charles P.

AU - Wan, Derrick C.

AU - Longaker, Michael T.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreERT2 recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.

AB - Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreERT2 recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.

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Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes

Abstract

ASJC Scopus subject areas

UN SDGs

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