Generation of IL-3–Secreting CD4+ T Cells by Microbial Challenge at Skin and Mucosal Barriers

Shajo Kunnath-Velayudhan, Michael F. Goldberg, Neeraj K. Saini, Tony W. Ng, Pooja Arora, Christopher T. Johndrow, Noemi Alejandra Saavedra-Avila, Alison J. Johnson, Jiayong Xu, John Kim, Nazanin Khajoueinejad, Christopher D. Petro, Betsy C. Herold, Gregoire Lauvau, John Chan, William R. Jacobs, Steven A. Porcelli

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


During Ag priming, naive CD4+ T cells differentiate into subsets with distinct patterns of cytokine expression that dictate to a major extent their functional roles in immune responses. We identified a subset of CD4+ T cells defined by secretion of IL-3 that was induced by Ag stimulation under conditions different from those associated with previously defined functional subsets. Using mouse models of bacterial and viral infections, we showed that IL-3–secreting CD4+ T cells were generated by infection at the skin and mucosa but not by infections introduced directly into the blood. Most IL-3–producing T cells coexpressed GM-CSF and other cytokines that define multifunctionality. Generation of IL-3–secreting T cells in vitro was dependent on IL-1 family cytokines and was inhibited by cytokines that induce canonical Th1 or Th2 cells. Our results identify IL-3–secreting CD4+ T cells as a potential functional subset that arises during priming of naive T cells in specific tissue locations.

Original languageEnglish (US)
Pages (from-to)161-171
Number of pages11
Issue number5
StatePublished - May 1 2019

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Allergy
  • Immunology


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