TY - JOUR
T1 - Functional cloning of genes regulating apoptosis in neuronal cells.
AU - Visconti, Roberta
AU - D'Adamio, Luciano
PY - 2007
Y1 - 2007
N2 - Here, we describe the use of a functional cloning approach, based on the screen of a genome-wide short hairpin RNA (shRNA) library, to identify novel genes regulating apoptosis in neuronal cells. Apoptosis is induced by doxorubicin and is detected with a fluorometric caspase 3 assay. Moreover, we describe also the screen of the library to identify genes regulating the processing f the beta-amyloid (Abeta) precursor protein (APP), the protein associated with the pathogenesis of lzheimer's disease. The levels of the peptide Abeta, produced by the APP processing, are detected with ELISA based on the innovative Delfia method developed by PerkinElmer.
AB - Here, we describe the use of a functional cloning approach, based on the screen of a genome-wide short hairpin RNA (shRNA) library, to identify novel genes regulating apoptosis in neuronal cells. Apoptosis is induced by doxorubicin and is detected with a fluorometric caspase 3 assay. Moreover, we describe also the screen of the library to identify genes regulating the processing f the beta-amyloid (Abeta) precursor protein (APP), the protein associated with the pathogenesis of lzheimer's disease. The levels of the peptide Abeta, produced by the APP processing, are detected with ELISA based on the innovative Delfia method developed by PerkinElmer.
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U2 - 10.1007/978-1-59745-504-6_9
DO - 10.1007/978-1-59745-504-6_9
M3 - Review article
C2 - 18309929
AN - SCOPUS:40949106390
SN - 1064-3745
VL - 399
SP - 125
EP - 131
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -