TY - JOUR
T1 - Functional analysis of histones H2A and H2B in transcriptional repression in Saccharomyces cerevisiae
AU - Recht, Judith
AU - Dunn, Barbara
AU - Raff, Amanda
AU - Osley, Mary Ann
PY - 1996
Y1 - 1996
N2 - The presence of H2A-H2B dimers in nucleosomes can inhibit the binding of transcription factors to chromatin templates. To study the roles of histones H2A and H2B in transcriptional repression in vivo, mutant forms of these histones were analyzed in two different assay systems. Two repression domains were identified in H2A. One domain includes residues that fall in the beginning of the H2A-H2B dimerization region, and the second is in the H2A N terminus, a region of potential interactions with nonhistone proteins. The function of H2A and H2B in one repression assay was found to be dependent on three SPT (suppressor of Ty) genes whose products are important for chromatin-mediated repression. These results suggest that repressive chromatin structure may be established through the interactions of the Spt proteins with these histones. In contrast, other proteins, the products of the HIR (histone regulation) genes, may function to direct H2A and H2B to specific promoters.
AB - The presence of H2A-H2B dimers in nucleosomes can inhibit the binding of transcription factors to chromatin templates. To study the roles of histones H2A and H2B in transcriptional repression in vivo, mutant forms of these histones were analyzed in two different assay systems. Two repression domains were identified in H2A. One domain includes residues that fall in the beginning of the H2A-H2B dimerization region, and the second is in the H2A N terminus, a region of potential interactions with nonhistone proteins. The function of H2A and H2B in one repression assay was found to be dependent on three SPT (suppressor of Ty) genes whose products are important for chromatin-mediated repression. These results suggest that repressive chromatin structure may be established through the interactions of the Spt proteins with these histones. In contrast, other proteins, the products of the HIR (histone regulation) genes, may function to direct H2A and H2B to specific promoters.
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U2 - 10.1128/MCB.16.6.2545
DO - 10.1128/MCB.16.6.2545
M3 - Article
C2 - 8649361
AN - SCOPUS:0029939581
SN - 0270-7306
VL - 16
SP - 2545
EP - 2553
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 6
ER -