Free 25(OH)D and calcium absorption, PTH, and markers of bone turnover

John Aloia, Ruban Dhaliwal, Mageda Mikhail, Albert Shieh, Alexandra Stolberg, Louis Ragolia, Melissa Fazzari, Steven A. Abrams

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Context: It has been proposed that serum free 25-hydroxyVitamin D [25(OH)D] may better reflect Vitamin D action than total 25(OH)D. An ELISA for serum free 25(OH)D has recently become available, permitting direct assay. Objective: To determine whether serum free 25(OH)D provides additional information in relation to calcium absorption and other biomarkers of vitaminDaction compared to total serum 25(OH)D. Setting: Ambulatory research setting in a teaching hospital. Outcome: Serum free 25(OH)D measured in a previously performed study of varied doses of Vitamin D3 (placebo and 800, 2000, and 4000 IU) on calcium absorption, PTH, procollagen type 1N-terminal propeptide, and C-terminal telopeptides of type I collagen. Free 25(OH)D was measured by ELISA. Calcium absorption was measured at baseline and at 10 weeks using stable dual calcium isotopes. Results: Seventy-one subjects completed this randomized, placebo-controlled trial. Baseline group meanfree and total 25(OH)D varied from 4.7±1.8 to 5.4±1.5 pg/mL, and from 23.7±5.9 to 25.9± 6.1 ng/mL, respectively. Participants assigned to the 4000-IU dose arm achieved free 25(OH)D levels of 10.4 pg/mL and total 25(OH)D levels of 40.4 ng/mL. Total and free 25(OH)D were highly correlated at baseline and after increasing Vitamin D dosing (r = 0.80 and 0.85, respectively). Free 25(OH)D closely reflected changes in total 25(OH)D. PTH was similarly correlated at baseline and follow-up with total and free 25(OH)D. Serum C-terminal telopeptides of type I collagen had a moderate positive correlation with total and free 25(OH)D at follow-up. The serum 1,25-dihydroxyvitaminDchange increased significantly with the change in 25(OH)D but not with the change in free 25(OH)D. Conclusion: There was no advantage from measuring free over total 25(OH)D in assessing the response of calcium absorption, PTH, and markers of bone turnover to Vitamin D. Free 25(OH)D responded to increasing doses of Vitamin D in a similar fashion to total 25(OH)D.

Original languageEnglish (US)
Pages (from-to)4140-4145
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Issue number11
StatePublished - Nov 2015
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


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