TY - JOUR
T1 - Fluorogenic assay for molecular typing of the Leishmania donovani complex
T2 - Taxonomic and clinical applications
AU - Quispe-Tintaya, Kelly Wilber
AU - Laurent, Thierry
AU - Decuypere, Saskia
AU - Hide, Mallorie
AU - Bañuls, Anne Laure
AU - De Doncker, Simonne
AU - Rijal, Suman
AU - Cañavate, Carmen
AU - Campino, Lenea
AU - Dujardin, Jean Claude
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/8/15
Y1 - 2005/8/15
N2 - We describe a new fluorogenic assay for the identification of species and intraspecies groups within the Leishmania donovani complex. The assay combined (1)2 polymerase chain reactions targeting the 2 cysteine proteinase b isogenes and (2) a fluorescence-resonance energy transfer/melting curve analysis of the polymorphisms within a 31-nt region. All strains within the L. donovani complex were distinguished from L. tropica, L. major, and L. aethiopica, and 5 distinct groups were identified within the L. donovani complex. Discrepancies were observed with the present taxonomy on the basis of isoenzyme analysis and concerned East African strains, which suggests the need for a systematic reevaluation of the taxonomy. The capacity to type parasites directly from clinical samples was demonstrated with blood and bone marrow samples. This rapid and high-throughput alternative for molecular diagnosis and epidemiological studies of visceral leishmaniasis could be adapted for use with other Leishmania species.
AB - We describe a new fluorogenic assay for the identification of species and intraspecies groups within the Leishmania donovani complex. The assay combined (1)2 polymerase chain reactions targeting the 2 cysteine proteinase b isogenes and (2) a fluorescence-resonance energy transfer/melting curve analysis of the polymorphisms within a 31-nt region. All strains within the L. donovani complex were distinguished from L. tropica, L. major, and L. aethiopica, and 5 distinct groups were identified within the L. donovani complex. Discrepancies were observed with the present taxonomy on the basis of isoenzyme analysis and concerned East African strains, which suggests the need for a systematic reevaluation of the taxonomy. The capacity to type parasites directly from clinical samples was demonstrated with blood and bone marrow samples. This rapid and high-throughput alternative for molecular diagnosis and epidemiological studies of visceral leishmaniasis could be adapted for use with other Leishmania species.
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U2 - 10.1086/432077
DO - 10.1086/432077
M3 - Article
C2 - 16028139
AN - SCOPUS:23244446793
SN - 0022-1899
VL - 192
SP - 685
EP - 692
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -
