TY - JOUR
T1 - Flow cytometry in hairy cell leukemia before and during interferon alfa‐2b therapy
AU - Vokes, Everett E.
AU - Bitter, Mitchell A.
AU - Ratain, Mark J.
AU - Prystowsky, Michael B.
AU - Daly, Karen
AU - Golomb, Harvey M.
PY - 1987/6/15
Y1 - 1987/6/15
N2 - Mononuclear cells from 15 patients with hairy cell leukemia were studied before and during therapy with interferon alfa‐2b (IFN) by regular peripheral blood differential counts and flow cytometry, using a panel of monoclonal antibodies (Moab). Seven leukemic phase patients (Group 1) had a mean leukocyte count of 48,870/μl at entry with a mean absolute hairy cell (HC) count of 40,100/μl. After 3 months on IFN, both parameters decreased significantly (WBC 3,500/μl; HC count 130/μl). In eight patients with a cytopenic form of the disease (Group 2) the mean leukocyte count rose from 2950/μl to 3890/μl while the mean absolute HC count decreased from 300/μl to 120/μl. The morphologic shifts correlated well with changes in the Moab reaction pattern. In Group 1 the activity of all Moab decreased significantly. In Group 2, only cells expressing Leu 3a and Leu 11a (a marker of natural killer cells) showed a significant shift, the latter increasing from 170/μl to 360/μl. This increase in natural killer cell antigen expression was not obvious based on routine morphologic observations alone. We show that flow cytometry may be a useful adjunct in monitoring the response of HCL to therapy. Changes in populations of cells that may be difficult to discriminate on morphologic grounds alone may be observed.
AB - Mononuclear cells from 15 patients with hairy cell leukemia were studied before and during therapy with interferon alfa‐2b (IFN) by regular peripheral blood differential counts and flow cytometry, using a panel of monoclonal antibodies (Moab). Seven leukemic phase patients (Group 1) had a mean leukocyte count of 48,870/μl at entry with a mean absolute hairy cell (HC) count of 40,100/μl. After 3 months on IFN, both parameters decreased significantly (WBC 3,500/μl; HC count 130/μl). In eight patients with a cytopenic form of the disease (Group 2) the mean leukocyte count rose from 2950/μl to 3890/μl while the mean absolute HC count decreased from 300/μl to 120/μl. The morphologic shifts correlated well with changes in the Moab reaction pattern. In Group 1 the activity of all Moab decreased significantly. In Group 2, only cells expressing Leu 3a and Leu 11a (a marker of natural killer cells) showed a significant shift, the latter increasing from 170/μl to 360/μl. This increase in natural killer cell antigen expression was not obvious based on routine morphologic observations alone. We show that flow cytometry may be a useful adjunct in monitoring the response of HCL to therapy. Changes in populations of cells that may be difficult to discriminate on morphologic grounds alone may be observed.
UR - http://www.scopus.com/inward/record.url?scp=0023181977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023181977&partnerID=8YFLogxK
U2 - 10.1002/1097-0142(19870615)59:12<1987::AID-CNCR2820591203>3.0.CO;2-K
DO - 10.1002/1097-0142(19870615)59:12<1987::AID-CNCR2820591203>3.0.CO;2-K
M3 - Article
C2 - 3494499
AN - SCOPUS:0023181977
SN - 0008-543X
VL - 59
SP - 1987
EP - 1991
JO - Cancer
JF - Cancer
IS - 12
ER -