Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3

Shreya Ahana Ayyub; S. L. Aswathy; Divya Dobriyal; Srinivas Aluri; Linda L. Spremulli; Umesh Varshney

doi:10.1016/j.mito.2017.08.006

Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3

Shreya Ahana Ayyub, S. L. Aswathy, Divya Dobriyal, Srinivas Aluri, Linda L. Spremulli, Umesh Varshney

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Initiation factor 3 (IF3) is a conserved translation factor. Mutations in mitochondrial IF3 (IF3_mt) have been implicated in disease pathology. Escherichia coli infCΔ55, compromised for IF3 activity, has provided an excellent heterologous system for IF3_mt structure-function analysis. IF3_mt allowed promiscuous initiation from AUA, AUU and ACG codons but avoided initiation with initiator tRNAs lacking the conserved 3GC pairs in their anticodon stems. Expression of IF3_mt N-terminal domain, or IF3_mt devoid of its typical N-, and C-terminal extensions improved fidelity of initiation in E. coli. The observations suggest that the IF3_mt terminal extensions relax the fidelity of translational initiation in mitochondria.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	Mitochondrion
Volume	39
DOIs	https://doi.org/10.1016/j.mito.2017.08.006
State	Published - Mar 2018
Externally published	Yes

Keywords

Fidelity
Initiation factor 3
Mitochondria
Protein synthesis
Ribosome

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Cell Biology

Access to Document

10.1016/j.mito.2017.08.006

Cite this

@article{46f1a2ee8b464434a23cf456cd2af225,

title = "Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3",

abstract = "Initiation factor 3 (IF3) is a conserved translation factor. Mutations in mitochondrial IF3 (IF3mt) have been implicated in disease pathology. Escherichia coli infCΔ55, compromised for IF3 activity, has provided an excellent heterologous system for IF3mt structure-function analysis. IF3mt allowed promiscuous initiation from AUA, AUU and ACG codons but avoided initiation with initiator tRNAs lacking the conserved 3GC pairs in their anticodon stems. Expression of IF3mt N-terminal domain, or IF3mt devoid of its typical N-, and C-terminal extensions improved fidelity of initiation in E. coli. The observations suggest that the IF3mt terminal extensions relax the fidelity of translational initiation in mitochondria.",

keywords = "Fidelity, Initiation factor 3, Mitochondria, Protein synthesis, Ribosome",

author = "Ayyub, {Shreya Ahana} and Aswathy, {S. L.} and Divya Dobriyal and Srinivas Aluri and Spremulli, {Linda L.} and Umesh Varshney",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V. and Mitochondria Research Society",

year = "2018",

month = mar,

doi = "10.1016/j.mito.2017.08.006",

language = "English (US)",

volume = "39",

pages = "1--8",

journal = "Mitochondrion",

issn = "1567-7249",

publisher = "Elsevier",

}

TY - JOUR

T1 - Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3

AU - Ayyub, Shreya Ahana

AU - Aswathy, S. L.

AU - Dobriyal, Divya

AU - Aluri, Srinivas

AU - Spremulli, Linda L.

AU - Varshney, Umesh

PY - 2018/3

Y1 - 2018/3

N2 - Initiation factor 3 (IF3) is a conserved translation factor. Mutations in mitochondrial IF3 (IF3mt) have been implicated in disease pathology. Escherichia coli infCΔ55, compromised for IF3 activity, has provided an excellent heterologous system for IF3mt structure-function analysis. IF3mt allowed promiscuous initiation from AUA, AUU and ACG codons but avoided initiation with initiator tRNAs lacking the conserved 3GC pairs in their anticodon stems. Expression of IF3mt N-terminal domain, or IF3mt devoid of its typical N-, and C-terminal extensions improved fidelity of initiation in E. coli. The observations suggest that the IF3mt terminal extensions relax the fidelity of translational initiation in mitochondria.

AB - Initiation factor 3 (IF3) is a conserved translation factor. Mutations in mitochondrial IF3 (IF3mt) have been implicated in disease pathology. Escherichia coli infCΔ55, compromised for IF3 activity, has provided an excellent heterologous system for IF3mt structure-function analysis. IF3mt allowed promiscuous initiation from AUA, AUU and ACG codons but avoided initiation with initiator tRNAs lacking the conserved 3GC pairs in their anticodon stems. Expression of IF3mt N-terminal domain, or IF3mt devoid of its typical N-, and C-terminal extensions improved fidelity of initiation in E. coli. The observations suggest that the IF3mt terminal extensions relax the fidelity of translational initiation in mitochondria.

KW - Fidelity

KW - Initiation factor 3

KW - Mitochondria

KW - Protein synthesis

KW - Ribosome

UR - http://www.scopus.com/inward/record.url?scp=85027517661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027517661&partnerID=8YFLogxK

U2 - 10.1016/j.mito.2017.08.006

DO - 10.1016/j.mito.2017.08.006

M3 - Article

C2 - 28804013

AN - SCOPUS:85027517661

SN - 1567-7249

VL - 39

SP - 1

EP - 8

JO - Mitochondrion

JF - Mitochondrion

ER -

Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this