TY - JOUR
T1 - Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
AU - Pennacchietti, Francesca
AU - Serebrovskaya, Ekaterina O.
AU - Faro, Aline R.
AU - Shemyakina, Irina I.
AU - Bozhanova, Nina G.
AU - Kotlobay, Alexey A.
AU - Gurskaya, Nadya G.
AU - Bodén, Andreas
AU - Dreier, Jes
AU - Chudakov, Dmitry M.
AU - Lukyanov, Konstantin A.
AU - Verkhusha, Vladislav V.
AU - Mishin, Alexander S.
AU - Testa, Ilaria
N1 - Funding Information:
We thank E. Uriati for help in recording data for the phototoxicity experiments. rsCherryRev1.4–vimentin and rsCherryRev–actinChromobody plasmids were a kind gift from S. Hell and S. Jakobs (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany). This work was supported by ERC starting grant MoNaLISA (https://doi. org/10.13039/501100000781; to I.T.), the Russian Science Foundation (grant 16-14-10364 to A.S.M. and I.I.S.), the US National Institutes of Health (grants GM122567 and NS103573 to V.V.V.), and the Brazilian CNPq (to A.R.F.). J.D. acknowledges the Carlsberg Foundation for support. Experiments were partially carried out with equipment provided by the CKP IBCH core facility, which is supported by Russian Ministry of Education and Science grant RFMEFI62117X0018.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
AB - Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.
UR - http://www.scopus.com/inward/record.url?scp=85049608980&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049608980&partnerID=8YFLogxK
U2 - 10.1038/s41592-018-0052-9
DO - 10.1038/s41592-018-0052-9
M3 - Article
C2 - 29988095
AN - SCOPUS:85049608980
SN - 1548-7091
VL - 15
SP - 601
EP - 604
JO - Nature Methods
JF - Nature Methods
IS - 8
ER -