TY - JOUR
T1 - Factors influencing plant regeneration from protoplasts isolated from long-term cell suspension culture of recalcitrant indica rice cultivar IR36
AU - Tang, Kexuan
AU - Hu, Quanan
AU - Zhao, Enpeng
AU - Wu, Aizhong
N1 - Funding Information:
The authors thank E. M. Southgate for critical reading of the manuscript. This research is partly supported by a grant from the Rockefeller Foundation.
PY - 2000
Y1 - 2000
N2 - Factors influencing successful establishment of embryogenic cell-suspension cultures and plant regeneration from long-term cell suspension-derived protoplasts of the recalcitrant Indica rice cultivar IR36 were studied. The factors included cell and protoplast culture medium, protoplast culture procedure, the source of nurse cells, and the regeneration procedure. Embryogenic cell suspension cultures could only be established from mature seed-derived callus of IR36 in AA-based medium (Muller and Grafe, 1978). Protoplast-derived colonies could be obtained only using the filter-membrane nurse-culture procedure when Lolium multiflorum suspension cells served as nurse, rather than wild rice (Oryza ridleyi) and Japonica rice (Oryza sativa cv. Taipei 309) cells. The utilization of a two-step regeneration procedure led to regeneration of fertile plants from protoplasts isolated from 2-yr-old cell suspensions of IR36, one of the most important but recalcitrant rice cultivars.
AB - Factors influencing successful establishment of embryogenic cell-suspension cultures and plant regeneration from long-term cell suspension-derived protoplasts of the recalcitrant Indica rice cultivar IR36 were studied. The factors included cell and protoplast culture medium, protoplast culture procedure, the source of nurse cells, and the regeneration procedure. Embryogenic cell suspension cultures could only be established from mature seed-derived callus of IR36 in AA-based medium (Muller and Grafe, 1978). Protoplast-derived colonies could be obtained only using the filter-membrane nurse-culture procedure when Lolium multiflorum suspension cells served as nurse, rather than wild rice (Oryza ridleyi) and Japonica rice (Oryza sativa cv. Taipei 309) cells. The utilization of a two-step regeneration procedure led to regeneration of fertile plants from protoplasts isolated from 2-yr-old cell suspensions of IR36, one of the most important but recalcitrant rice cultivars.
KW - Culture medium
KW - IR36
KW - Membrane-filter nurse culture
KW - Two-step regeneration
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U2 - 10.1007/s11627-000-0047-x
DO - 10.1007/s11627-000-0047-x
M3 - Article
AN - SCOPUS:0033797362
SN - 1054-5476
VL - 36
SP - 255
EP - 259
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
IS - 4
ER -