Eye development

Nicholas E. Baker; Ke Li; Manon Quiquand; Robert Ruggiero; Lan Hsin Wang

doi:10.1016/j.ymeth.2014.04.007

Eye development

Nicholas E. Baker, Ke Li, Manon Quiquand, Robert Ruggiero, Lan Hsin Wang

Genetics

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The eye has been one of the most intensively studied organs in Drosophila. The wealth of knowledge about its development, as well as the reagents that have been developed, and the fact that the eye is dispensable for survival, also make the eye suitable for genetic interaction studies and genetic screens. This article provides a brief overview of the methods developed to image and probe eye development at multiple developmental stages, including live imaging, immunostaining of fixed tissues, in situ hybridizations, and scanning electron microscopy and color photography of adult eyes. Also summarized are genetic approaches that can be performed in the eye, including mosaic analysis and conditional mutation, gene misexpression and knockdown, and forward genetic and modifier screens.

Original language	English (US)
Pages (from-to)	252-259
Number of pages	8
Journal	Methods
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2014.04.007
State	Published - Jun 15 2014

Keywords

Drosophila eye
Genetic screen
Immunocytochemistry
In situ hybridization
Live imaging
Scanning electron microscopy

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.ymeth.2014.04.007

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title = "Eye development",

abstract = "The eye has been one of the most intensively studied organs in Drosophila. The wealth of knowledge about its development, as well as the reagents that have been developed, and the fact that the eye is dispensable for survival, also make the eye suitable for genetic interaction studies and genetic screens. This article provides a brief overview of the methods developed to image and probe eye development at multiple developmental stages, including live imaging, immunostaining of fixed tissues, in situ hybridizations, and scanning electron microscopy and color photography of adult eyes. Also summarized are genetic approaches that can be performed in the eye, including mosaic analysis and conditional mutation, gene misexpression and knockdown, and forward genetic and modifier screens.",

keywords = "Drosophila eye, Genetic screen, Immunocytochemistry, In situ hybridization, Live imaging, Scanning electron microscopy",

author = "Baker, {Nicholas E.} and Ke Li and Manon Quiquand and Robert Ruggiero and Wang, {Lan Hsin}",

note = "Funding Information: We thank Andreas Jenny for providing Fig. 1 B and John Fullard for reading the manuscript. Supported by grants from the NIH ( GM047892 and EY021614 ), by an unrestricted grant from Research to Prevent Blindness to the Department of Ophthalmology and Visual Sciences, and by a 2012 Established Investigator Award from Research to Prevent Blindness to NEB. ",

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AU - Baker, Nicholas E.

AU - Li, Ke

AU - Quiquand, Manon

AU - Ruggiero, Robert

AU - Wang, Lan Hsin

N1 - Funding Information: We thank Andreas Jenny for providing Fig. 1 B and John Fullard for reading the manuscript. Supported by grants from the NIH ( GM047892 and EY021614 ), by an unrestricted grant from Research to Prevent Blindness to the Department of Ophthalmology and Visual Sciences, and by a 2012 Established Investigator Award from Research to Prevent Blindness to NEB.

PY - 2014/6/15

Y1 - 2014/6/15

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AB - The eye has been one of the most intensively studied organs in Drosophila. The wealth of knowledge about its development, as well as the reagents that have been developed, and the fact that the eye is dispensable for survival, also make the eye suitable for genetic interaction studies and genetic screens. This article provides a brief overview of the methods developed to image and probe eye development at multiple developmental stages, including live imaging, immunostaining of fixed tissues, in situ hybridizations, and scanning electron microscopy and color photography of adult eyes. Also summarized are genetic approaches that can be performed in the eye, including mosaic analysis and conditional mutation, gene misexpression and knockdown, and forward genetic and modifier screens.

KW - Drosophila eye

KW - Genetic screen

KW - Immunocytochemistry

KW - In situ hybridization

KW - Live imaging

KW - Scanning electron microscopy

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JO - Methods

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Eye development

Abstract

Keywords

ASJC Scopus subject areas

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