TY - JOUR
T1 - Evidence that a single replication fork proceeds from early to late replicating domains in the IgH locus in a non-B cell line
AU - Ermakova, Olga V.
AU - Nguyen, Lam H.
AU - Little, Randall D.
AU - Chevillard, Christophe
AU - Riblet, Roy
AU - Ashouian, Nasrin
AU - Birshtein, Barbara K.
AU - Schildkraut, Carl L.
N1 - Funding Information:
This work was supported by National Institutes of Health grants GM45751 (C. L. S.), AI13509 (B. K. B.), and AI23548 (R. R.). Support was also provided by Cancer Center Core Grant NIH/NCI P30-CA13330. L. H. N. was supported by a fellowship from the Leukemia Society of America. We thank Drs. Paolo Norio, Peter Dijkwel, David Gilbert, and James Borowiec for critical reading of the manuscript. This study is dedicated to the memory of Dr. Ruth M. Kavenoff whose exquisite EM images of DNA will continue to enlighten.
PY - 1999/3
Y1 - 1999/3
N2 - In non-B cell lines, like the murine erythroleukemia cell line (MEL), the most distal IgH constant region gene, Cα, replicates early in S; other heavy chain constant region genes, joining and diversity segments, and the most proximal Vh gene replicate successively later in S in a 3' to 5' direction proportional to their distance from Cα. In MEL, replication forks detected in the IgH locus also proceed in the same 3' to 5' direction for ~400 kb, beginning downstream of the IgH 3' regulatory region and continuing to the D region, as well as within the Vh81X gene. Downstream of the initiation region is an early replicating domain, and upstream of Vh81X is a late replicating domain. Hence, the gradual transition between early and late replicated domains can be achieved by a single replication fork.
AB - In non-B cell lines, like the murine erythroleukemia cell line (MEL), the most distal IgH constant region gene, Cα, replicates early in S; other heavy chain constant region genes, joining and diversity segments, and the most proximal Vh gene replicate successively later in S in a 3' to 5' direction proportional to their distance from Cα. In MEL, replication forks detected in the IgH locus also proceed in the same 3' to 5' direction for ~400 kb, beginning downstream of the IgH 3' regulatory region and continuing to the D region, as well as within the Vh81X gene. Downstream of the initiation region is an early replicating domain, and upstream of Vh81X is a late replicating domain. Hence, the gradual transition between early and late replicated domains can be achieved by a single replication fork.
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U2 - 10.1016/S1097-2765(00)80459-1
DO - 10.1016/S1097-2765(00)80459-1
M3 - Article
C2 - 10198634
AN - SCOPUS:0033104977
SN - 1097-2765
VL - 3
SP - 321
EP - 330
JO - Molecular Cell
JF - Molecular Cell
IS - 3
ER -