Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

Curtis F. Brewer; John P. Riehm

doi:10.1016/0003-2697(67)90007-3

Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

Curtis F. Brewer, John P. Riehm

Research output: Contribution to journal › Article › peer-review

221 Scopus citations

Abstract

The reaction between NEM and the two proteins lysozyme and ribonuclease A results, under certain experimental conditions, in the alkylation of ε{lunate}-amino and imidazole groups. The extent of reaction may be determined by amino acid analysis since the reaction products yield ninhydrin-positive zones.

Original language	English (US)
Pages (from-to)	248-255
Number of pages	8
Journal	Analytical Biochemistry
Volume	18
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(67)90007-3
State	Published - Feb 1967
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins",

abstract = "The reaction between NEM and the two proteins lysozyme and ribonuclease A results, under certain experimental conditions, in the alkylation of ε{lunate}-amino and imidazole groups. The extent of reaction may be determined by amino acid analysis since the reaction products yield ninhydrin-positive zones.",

author = "Brewer, {Curtis F.} and Riehm, {John P.}",

year = "1967",

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doi = "10.1016/0003-2697(67)90007-3",

language = "English (US)",

volume = "18",

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journal = "Analytical Biochemistry",

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T1 - Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

AU - Brewer, Curtis F.

AU - Riehm, John P.

PY - 1967/2

Y1 - 1967/2

N2 - The reaction between NEM and the two proteins lysozyme and ribonuclease A results, under certain experimental conditions, in the alkylation of ε{lunate}-amino and imidazole groups. The extent of reaction may be determined by amino acid analysis since the reaction products yield ninhydrin-positive zones.

AB - The reaction between NEM and the two proteins lysozyme and ribonuclease A results, under certain experimental conditions, in the alkylation of ε{lunate}-amino and imidazole groups. The extent of reaction may be determined by amino acid analysis since the reaction products yield ninhydrin-positive zones.

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U2 - 10.1016/0003-2697(67)90007-3

DO - 10.1016/0003-2697(67)90007-3

M3 - Article

AN - SCOPUS:0000836690

SN - 0003-2697

VL - 18

SP - 248

EP - 255

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

Abstract

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