TY - JOUR
T1 - Evaluation of potential antigenotoxic, cytotoxic and proapoptotic effects of the olive oil by-product "alperujo", hydroxytyrosol, tyrosol and verbascoside
AU - Anter, Jaouad
AU - Tasset, Inmaculada
AU - Demyda-Peyrás, Sebastián
AU - Ranchal, Isidora
AU - Moreno-Millán, Miguel
AU - Romero-Jimenez, Magdalena
AU - Muntané, Jordi
AU - Luque de Castro, María Dolores
AU - Muñoz-Serrano, Andrés
AU - Alonso-Moraga, Ángeles
N1 - Funding Information:
This work was supported by the Consejería de Innovación, Ciencia y Empresa ( P06-FQM-01515 ) and Consejería de Salud ( SAS 0103/2006 ) both from Junta de Andalucía (Spain). We thank Mr. Cristobal Medina Raso by the color artwork.
PY - 2014/9/15
Y1 - 2014/9/15
N2 - Olive oil is an integral ingredient of the "Mediterranean diet". The olive oil industry generates large quantities of a by-product called "alperujo" (AL) during the two-phase centrifugation system developed in the early nineties. AL could be a potent exploitable source of natural phenolic antioxidants. Our results showed that AL and its distinctive phenols hydroxytyrosol, tyrosol and verbascoside were not genotoxic in the Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster and exerted antigenotoxic activity against DNA oxidative damage generated by hydrogen peroxide (H2O2). Alperujo and hydroxytyrosol also exhibited notable antiproliferative and caspase 3-dependent proapoptotic effects toward the human tumoral cell line HL60. AL can provide a cheap and efficient source of chemopreventive phenolic compounds with strong antioxidant properties, becoming a promising and potent therapeutic drug in the future.
AB - Olive oil is an integral ingredient of the "Mediterranean diet". The olive oil industry generates large quantities of a by-product called "alperujo" (AL) during the two-phase centrifugation system developed in the early nineties. AL could be a potent exploitable source of natural phenolic antioxidants. Our results showed that AL and its distinctive phenols hydroxytyrosol, tyrosol and verbascoside were not genotoxic in the Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster and exerted antigenotoxic activity against DNA oxidative damage generated by hydrogen peroxide (H2O2). Alperujo and hydroxytyrosol also exhibited notable antiproliferative and caspase 3-dependent proapoptotic effects toward the human tumoral cell line HL60. AL can provide a cheap and efficient source of chemopreventive phenolic compounds with strong antioxidant properties, becoming a promising and potent therapeutic drug in the future.
KW - Alperujo
KW - Apoptosis
KW - Drosophila
KW - HL60
KW - Phenolic antioxidants
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U2 - 10.1016/j.mrgentox.2014.07.002
DO - 10.1016/j.mrgentox.2014.07.002
M3 - Article
C2 - 25308544
AN - SCOPUS:84906264348
SN - 1383-5718
VL - 772
SP - 25
EP - 33
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
ER -