Ephrin-B1 and ephrin-B2 mediate EphB-dependent presynaptic development via syntenin-1

Andrew C. McClelland, Sean I. Sheffler-Collins, Matthew S. Kayser, Matthew B. Dalva

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


The development of central nervous system synapses requires precise coordination between presynaptic and postsynaptic components. The EphB family controls postsynaptic development by interacting with glutamate receptors and regulating dendritic filopodia motility, but how EphBs induce the formation of presynaptic specializations is less well understood. Here, we show that knockdown of presynaptic ephrin-B1, ephrin-B2, or syntenin-1, but not ephrin-B3, prevents EphB-dependent presynaptic development. Ephrin-B1, ephrin-B2, and syntenin-1 are clustered together with presynaptic markers, suggesting that these molecules function jointly in presynaptic development. Knockdown of ephrin-B1 or ephrin-B2 reduces the number of synaptic specializations and the colocalization of syntenin-1 with synaptic markers. Simultaneous knockdown of ephrin-B1 and ephrin-B2 suggests that they function independently in the formation of synaptic contacts, but act together to recruit syntenin-1 to presynaptic terminals. Taken together, these results demonstrate that ephrin-B1 and ephrin-B2 function with EphB to mediate presynaptic development via syntenin-1.

Original languageEnglish (US)
Pages (from-to)20487-20492
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number48
StatePublished - Dec 1 2009
Externally publishedYes


  • Coculture
  • Cortex
  • Eph
  • Excitatory synapse
  • Synaptogenesis

ASJC Scopus subject areas

  • General


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