TY - JOUR
T1 - Enzymatic analysis of total- and HDL-cholesterol
T2 - Comparison with the standardized Liebermann-Burchard method used by the lipid research clinics program
AU - Bachorik, Paul S.
AU - Virgil, Donna G.
AU - Derby, Carol
AU - Widman, David
AU - McMahon, Robert
AU - Fulwood, Robinson P.
AU - Ezzati, Trena
N1 - Funding Information:
This work was supportedb y NIH ContractN o. l-HVl-2158, NIH l-31450-03,a nd NIH contractN Ol-HV-12243-L.
PY - 1988/6/15
Y1 - 1988/6/15
N2 - We compared two enzymatic cholesterol methods with the standardized chemical method used in the Lipid Research Clinic's (LRC) program. The methods were used to measure total cholesterol and high density lipoprotein (HDL) cholesterol in heparin-MnCl2 supernatants of 1812 sera collected over a 16-mth period from subjects who were sampled as part of the Hispanic Health and Nutrition Examination Survey. Thirty percent of the subjects had fasted for 12 h or more before venepuncture. The enzymatic total cholesterol values were 1.4-1.8% lower than the LRC method and both enzymatic methods correlated highly with the LRC method (r > 0.97). The enzymatic HDL cholesterol values were 2.4 and 6.4% higher than the LRC method, and the correlation between the enzymatic and LRC methods was > 0.93. The differences between the enzymatic and LRC methods were the same in samples from fasting and non-fasting subjects.
AB - We compared two enzymatic cholesterol methods with the standardized chemical method used in the Lipid Research Clinic's (LRC) program. The methods were used to measure total cholesterol and high density lipoprotein (HDL) cholesterol in heparin-MnCl2 supernatants of 1812 sera collected over a 16-mth period from subjects who were sampled as part of the Hispanic Health and Nutrition Examination Survey. Thirty percent of the subjects had fasted for 12 h or more before venepuncture. The enzymatic total cholesterol values were 1.4-1.8% lower than the LRC method and both enzymatic methods correlated highly with the LRC method (r > 0.97). The enzymatic HDL cholesterol values were 2.4 and 6.4% higher than the LRC method, and the correlation between the enzymatic and LRC methods was > 0.93. The differences between the enzymatic and LRC methods were the same in samples from fasting and non-fasting subjects.
KW - Cholesterol measurement
KW - Enzymatic methods
KW - HDl-cholesterol measurements
UR - http://www.scopus.com/inward/record.url?scp=0023934920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023934920&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(88)90057-5
DO - 10.1016/0009-8981(88)90057-5
M3 - Article
C2 - 3390958
AN - SCOPUS:0023934920
SN - 0009-8981
VL - 174
SP - 307
EP - 314
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 3
ER -