Enterotoxic Effect of Stool Supernatant of Cryptosporidium–infected Calves on Human Jejunum

Douglas Simon, Jonathan David, Lawrence J. Brandt

Research output: Contribution to journalReview articlepeer-review

4 Scopus citations


The authors infected calves with C. parvum, collected diarrheal stools, centrifuged the stool, and then filtered the supernatant. Microbiological studies detected Cryptospo‐ridium oocysts in the calves' diarrhea but not in the filtered supernatant. The authors placed the supernatant on either the mucosa or serosal side of strips of human jejunum mounted in Ussing chambers. Stools collected from the calves before infection with Cryptosporidia were also centrifuged and filtered, and these supematants served as controls. Stool supernatant from the Cryptosporidia‐m ‐ fected calves, when added to the mucosal side of the jejunum, caused a significant increase in short circuit current but no change on tissue conductance. This effect was dose–dependent, reversible, calcium– and chloride–dependent, and lost on heating the supernatant No short circuit current changes were noted when infected stools were added to the serosal side of the jejunum, or when the control supernatant was added to either mucosa or serosa. Histological examination of the human jejunal sections revealed a mild and nonspecific inflammation. The authors concluded that there is enterotoxic activity in the stools of calves infected with Cryptospordium parvum, and that this enterotoxic activity may be responsible for secretory diarrhea in humans.

Original languageEnglish (US)
Pages (from-to)2277-2278
Number of pages2
JournalThe American Journal of Gastroenterology
Issue number12
StatePublished - Dec 1994

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology


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