TY - JOUR
T1 - Enkephalin convertase
T2 - Purification and characterization of a specific enkephalin-synthesizing carboxypeptidase localized to adrenal chromaffin granules
AU - Fricker, L. D.
AU - Snyder, S. H.
PY - 1982
Y1 - 1982
N2 - A specific carboxypeptidase that converts enkephalin precursors into enkephalin in adrenal chromaffin granule has been purified and characterized. In the adrenal this enzyme, designated enkephalin convertase, is uniquely localized to the chromaffin granules, which contain enkephalin and precursor peptides. Enkephalin convertase is markedly stimulated by CoCl2 and inhibited by EDTA or 1,10-phenanthroline, unlike the lysosomal carboxypeptidase. The purified enzyme has a high affinity for the hexapeptides [Met5]- and [Leu5]enkephalin-Arg6 (51 and 83 μM, respectively) and a somewhat lower affinity for the hexapeptides [Met5]- and [Leu5]enkephalin-Lys6 (195 and 174 μM). Brain enkephalin convertase shows 10-fold regional variations, unlike other carboxypeptidases, which are uniformly distributed. Enkephalin convertase appears to be associated selectively and physiologically with biosynthesis of the enkephalins.
AB - A specific carboxypeptidase that converts enkephalin precursors into enkephalin in adrenal chromaffin granule has been purified and characterized. In the adrenal this enzyme, designated enkephalin convertase, is uniquely localized to the chromaffin granules, which contain enkephalin and precursor peptides. Enkephalin convertase is markedly stimulated by CoCl2 and inhibited by EDTA or 1,10-phenanthroline, unlike the lysosomal carboxypeptidase. The purified enzyme has a high affinity for the hexapeptides [Met5]- and [Leu5]enkephalin-Arg6 (51 and 83 μM, respectively) and a somewhat lower affinity for the hexapeptides [Met5]- and [Leu5]enkephalin-Lys6 (195 and 174 μM). Brain enkephalin convertase shows 10-fold regional variations, unlike other carboxypeptidases, which are uniformly distributed. Enkephalin convertase appears to be associated selectively and physiologically with biosynthesis of the enkephalins.
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U2 - 10.1073/pnas.79.12.3886
DO - 10.1073/pnas.79.12.3886
M3 - Article
C2 - 6808517
AN - SCOPUS:0001727492
SN - 0027-8424
VL - 79
SP - 3886
EP - 3890
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12 I
ER -