TY - JOUR
T1 - Elevated GM2 ganglioside is associated with dendritic proliferation in normal developing neocortex
AU - Goodman, Linda A.
AU - Walkley, Steven U.
N1 - Funding Information:
This work was supported by National Institutes of Health Training Grant NS 07098 (L.A.G.) and NS 18804 (S.U.W.). We thank Sarah Wurzelmann and May Huang for their expert technical assistance. We thank Drs. Donald A. Siegel, Kostantin Dobrenis, and Joseph F. Cubells for their helpful comments on the manuscript. We are grateful to Dr. Philip O. Livingston of Memorial Sloan-Kettering Cancer Center for providing the monoclonal antibody to GM2 ganglioside, and thank Dr. Dennis W. Dickson of Albert Einstein College of Medicine for contributing the antibody, Alz 50.
PY - 1996/5/31
Y1 - 1996/5/31
N2 - Mature pyramidal neurons of cerebral cortex in several neuronal storage diseases elaborate ectopic dendrites. These dendrites appear specifically on pyramidal neurons containing elevated GM2 ganglioside and a variety of studies support the hypothesis that this ganglioside is responsible for inducing the new dendrite growth. To determine whether a similar association between GM2 ganglioside and dendrite growth occurs in normal neurons, we used an antibody to localize GM2 in developing cat neocortex. Our results show that GM2 ganglioside is elevated in normal cortical neurons during the period when dendritogenesis is occurring, but is greatly diminished in these cells after dendritic differentiation is complete. Elevations of GM2 occur in deep neurons earlier than in superficial ones, a sequence that corresponds closely to the inside-first, outside-last progression of cortical neuron differentiation. Ultrastructurally, GM2 immunoreactivity is found sequestered in vesicles with a distribution that coincides with sites of ganglioside synthesis and transport. The close association between elevated GM2 ganglioside and dendrite growth-in cortical pyramidal neurons during normal development, coupled with a similar correlation between GM2 and ectopic dendritogenesis in neuronal storage diseases,support the view that this specific ganglioside plays a pivotal role in regulating dendritogenesis.
AB - Mature pyramidal neurons of cerebral cortex in several neuronal storage diseases elaborate ectopic dendrites. These dendrites appear specifically on pyramidal neurons containing elevated GM2 ganglioside and a variety of studies support the hypothesis that this ganglioside is responsible for inducing the new dendrite growth. To determine whether a similar association between GM2 ganglioside and dendrite growth occurs in normal neurons, we used an antibody to localize GM2 in developing cat neocortex. Our results show that GM2 ganglioside is elevated in normal cortical neurons during the period when dendritogenesis is occurring, but is greatly diminished in these cells after dendritic differentiation is complete. Elevations of GM2 occur in deep neurons earlier than in superficial ones, a sequence that corresponds closely to the inside-first, outside-last progression of cortical neuron differentiation. Ultrastructurally, GM2 immunoreactivity is found sequestered in vesicles with a distribution that coincides with sites of ganglioside synthesis and transport. The close association between elevated GM2 ganglioside and dendrite growth-in cortical pyramidal neurons during normal development, coupled with a similar correlation between GM2 and ectopic dendritogenesis in neuronal storage diseases,support the view that this specific ganglioside plays a pivotal role in regulating dendritogenesis.
KW - Dendrite growth
KW - Ganglioside function
KW - Ganglioside localization
KW - Neocortical development
KW - Pyramidal cell differentiation
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U2 - 10.1016/0165-3806(96)00029-6
DO - 10.1016/0165-3806(96)00029-6
M3 - Article
C2 - 8804703
AN - SCOPUS:0029979874
SN - 0165-3806
VL - 93
SP - 162
EP - 171
JO - Developmental Brain Research
JF - Developmental Brain Research
IS - 1-2
ER -