EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells

Jacco Van Rheenen, Xiaoyan Song, Wies Van Roosmalen, Michael Cammer, Xiaoming Chen, Vera DesMarais, Shu Chin Yip, Jonathan M. Backer, Robert J. Eddy, John S. Condeelis

Research output: Contribution to journalArticlepeer-review

201 Scopus citations


Lamellipodial protrusion and directional migration of carcinoma cells towards chemoattractants, such as epidermal growth factor (EGF), depend upon the spatial and temporal regulation of actin cytoskeleton by actin-binding proteins (ABPs). It is generally hypothesized that the activity of many ABPs are temporally and spatially regulated by PIP2; however, this is mainly based on in vitro-binding and structural studies, and generally in vivo evidence is lacking. Here, we provide the first in vivo data that directly visualize the spatial and temporal regulation of cofilin by PIP2 in living cells. We show that EGF induces a rapid loss of PIP2 through PLC activity, resulting in a release and activation of a membrane-bound pool of cofilin. Upon release, we find that cofilin binds to and severs F-actin, which is coincident with actin polymerization and lamellipod formation. Moreover, our data provide evidence for how PLC is involved in the formation of protrusions in breast carcinoma cells during chemotaxis and metastasis towards EGF.

Original languageEnglish (US)
Pages (from-to)1247-1259
Number of pages13
JournalJournal of Cell Biology
Issue number6
StatePublished - Dec 17 2007

ASJC Scopus subject areas

  • Cell Biology


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