Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves

W. Ray Waters, Mitchell V. Palmer, Brian J. Nonnecke, Tyler C. Thacker, Charles F.Capinos Scherer, D. Mark Estes, R. Glyn Hewinson, H. Martin Vordermeier, S. Whitney Barnes, Glenn C. Federe, John R. Walker, Richard J. Glynne, Tsungda Hsu, Brian Weinrick, Karolin Biermann, Michelle H. Larsen, William R. Jacobs

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


An attenuated Mycobacterium bovis RD1 deletion (ΔRD1) mutant of the Ravenel strain was constructed, characterized, and sequenced. This M. bovis ΔRD1 vaccine strain administered to calves at 2 weeks of age provided similar efficacy as M. bovis bacillus Calmette Guerin (BCG) against low dose, aerosol challenge with virulent M. bovis at 3.5 months of age. Approximately 4.5 months after challenge, both ΔRD1- and BCG-vaccinates had reduced tuberculosis (TB)-associated pathology in lungs and lung-associated lymph nodes and M. bovis colonization of tracheobronchial lymph nodes as compared to non-vaccinates. Mean central memory responses elicited by either ΔRD1 or BCG prior to challenge correlated with reduced pathology and bacterial colonization. Neither ΔRD1 or BCG elicited IFN-γ responses to rESAT-6:CFP-10 prior to challenge, an emerging tool for modern TB surveillance programs. The ΔRD1 strain may prove useful for bovine TB vaccine programs, particularly if additional mutations are included to improve safety and immunogenicity.

Original languageEnglish (US)
Pages (from-to)1201-1209
Number of pages9
Issue number8
StatePublished - Feb 18 2009


  • Aerosol inoculation
  • BCG
  • Live bacterial vaccine
  • Neonatal calf
  • RD1
  • Tuberculosis

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases


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