Effects of short-term and long-term androgen treatment on the diaphragm in male and female rats

D. J. Prezant, D. E. Valentine, E. I. Gentry, B. Richner, J. Cahill, K. Freeman

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


The effects of short-term (2.5 wk) and long-term (10 wk) testosterone propionate (2.5 mg/day; 5 days/wk) treatment on diaphragm contractility, fatigue resistance, and fiber type proportions were studied in male and female rats. Contractility and fatigue resistance indexes were measured in an in vitro diaphragm costal strip preparation by direct stimulation at 37°C. The fatigue paradigm consisted of 30 trains/min at 5 Hz (50% duty cycle) for 10 min. Fatigue resistance indexes were calculated as postfatigue divided by baseline forces. In females but not males, testosterone treatment produced significant increases in body weight, costal diaphragm weight, and contractility and significant decreases in fatigue resistance indexes. The interaction between testosterone treatment and the duration of treatment was significant, with the increase in contractility (females) being significant after short-term but not long-term treatment. No significant difference in fiber type proportions or areas was observed, regardless of treatment duration or the preexperimental, basal circulating level of androgen.

Original languageEnglish (US)
Pages (from-to)1140-1149
Number of pages10
JournalJournal of applied physiology
Issue number3
StatePublished - 1993


  • androgens
  • respiratory muscles
  • sex differences

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Effects of short-term and long-term androgen treatment on the diaphragm in male and female rats'. Together they form a unique fingerprint.

Cite this