Effects of manganese (Mn) on the developing rat brain: Oxidative-stress related endpoints

Sarah Weber, David C. Dorman, Lawrence H. Lash, Keith Erikson, Kent E. Vrana, Michael Aschner

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


We evaluated biochemical endpoints related to oxidative stress in brains of neonatal rats exposed to manganese (Mn). Oral Mn chloride (MnCl2) (0, 25, or 50 mg Mn chloride kg-1 body weight per day) was given daily to neonatal rats throughout lactation (i.e. from postnatal day (PND) 1 to 21). As previously reported by [J. Appl. Toxicol. 20 (2000) 179], this treatment paradigm results in increased cerebral cortex (CTX) Mn concentrations in PND 21 rats from both Mn treatment groups. High dose Mn exposure also results in increased cerebellar Mn concentrations. This preliminary study determined whether this exposure paradigm also affects cerebrocortical or cerebellar metallothionein (MT) mRNA levels, glutamine synthetase (GS) activity, GS protein levels, as well as total glutathione (GSH) levels. High dose Mn exposure significantly increased (P < 0.05) total cerebrocortical GSH without accompanying changes in any of the other measured parameters. Therefore, it is unlikely that high dose Mn exposure is associated with oxidative stress in this experimental paradigm.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
Issue number2
StatePublished - Jul 2002
Externally publishedYes


  • Manganese
  • Neurotoxicity
  • Oxidative stress

ASJC Scopus subject areas

  • General Neuroscience
  • Toxicology


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