Effects of high-impact mechanical loading on synovial cell cultures

Irene Sun, Yunlong Liu, Shigeo M. Tanaka, Chung W. Lee, Hui Bin Sun, Hiroki Yokota

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Cartilage metabolism in response to mechanical loading is an important subject in sports science and medicine. In animal studies high-impact exercise is known to stimulate bone adaptation and increase bone mass. However, mechanical impacts potentially induce tissue swelling and occasionally degradation of connective tissues in synovium and articular cartilage. These detrimental outcomes should be properly evaluated clinically and biochemically. Using two synovial cell cultures derived from normal and rheumatic tissues, we examined the biochemical effects of impulsive mechanical loads on expression and activities of influential proteolytic enzymes in joints, matrix metalloproteinases (MMPs), and their natural inhibitors, tissue inhibitors of metalloproteinases (TIMPs). The molecular analysis demonstrates that an impact factor (I m), the ratio of the maximum force to weight, served as a good indicator for assessment of the inflammatory responses. The results showed that high impact above I m = 40 to 80 elevated not only expression but also enzymatic activities of MMPs.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalJournal of Sports Science and Medicine
Issue number1
StatePublished - 2004
Externally publishedYes


  • Impulsive factor
  • MMP
  • Rheumatoid arthritis
  • Synovium

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation


Dive into the research topics of 'Effects of high-impact mechanical loading on synovial cell cultures'. Together they form a unique fingerprint.

Cite this