Effect of Lactobacillus plantarum and l-arginine against endotoxin-induced liver injury in a rat model

Praveen Rishi, Sushma Bharrhan, Gurdeep Singh, Indu Pal Kaur

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Aims: Use of probiotics, alone or as adjunct to other established therapies, has been reported to have potential benefits. Recently, we have reported protective potential of probiotic against Salmonella-induced liver injury. However, co-supplementation with prebiotics did not result in meaningful synergism at systemic level. Owing to the action of probiotics at the mucosal level and of arginine at systemic level, the present study was designed to evaluate the effect of Lactobacillus plantarum alone or in conjunction with arginine to combat endotoxin-mediated liver injury in rats. Main methods: Bacterial endotoxin/lipopolysaccharide (LPS) was injected intraperitoneally and animals were sacrificed 8 h post-challenge. Efficacy of L. plantarum alone or in conjunction with l-arginine was determined on the basis of enzyme markers, histology, levels of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) in addition to identification of amino acids by paper chromatography. Key findings: Prior supplementation of LPS-challenged rats with L. plantarum (10 10 CFU per rat given orally for 10 days) demonstrated decreased levels of liver enzymes, NO and TNF-α. Interestingly, complementing Lactobacillus with arginine revealed a synergistic decrease not only in the liver markers but also in NO and TNF-α along with increased intensity of ornithine and methionine. Histological evidence also confirmed the protective efficacy of probiotic in conjunction with l-arginine. Significance: Presence of ornithine and methionine in the probiotic-arginine co-supplemented group suggests involvement of arginase-induced synthesis of polyamines. This study highlights that L. plantarum may direct l-arginine metabolism towards polyamine synthesis thereby exhibiting synergistic effect against liver injury.

Original languageEnglish (US)
Pages (from-to)847-853
Number of pages7
JournalLife Sciences
Issue number23-24
StatePublished - Dec 5 2011
Externally publishedYes


  • Endotoxin
  • Lactobacillus plantarum
  • Liver injury
  • Nitric oxide
  • Polyamines
  • Probiotic
  • l-arginine

ASJC Scopus subject areas

  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry, Genetics and Molecular Biology


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