@article{dfa4e98b1d0f4c0f9746c110ce92b13e,
title = "During the early prediabetic period in NOD mice, the pathogenic CD8+ T-cell population comprises multiple antigenic specificities",
abstract = "In the NOD mouse model of type 1 diabetes, major histocompatibility complex (MHC) class I-restricted CD8+ T cells are essential for disease development. However, the extent of diversity of their antigenic specificities during early pathogenesis remains unclear. An insulin-derived peptide was recently identified as the epitope for the NOD-derived diabetogenic T-cell clone G9C8. To explore the possibility that the early pathogenic CD8+ T-cell population comprises additional antigenic specificities, we employed the T-cell clones AI4 and NY8.3, both of which are pathogenic and represent specificities present in early insulitic lesions. The clones responded to distinct fractions of chromatographically separated class I MHC-bound peptides purified from NOD-derived NIT-1 β cells, and neither clone recognized the insulin-derived peptide. NIT-1 cells represent an unlimited peptide source that will allow for the future isolation and sequencing of the novel multiple epitopes targeted early in the autoimmune response by pathogenic CD8+ T cells.",
keywords = "Autoimmunity, CD8 T cells, Epitopes, NOD mice, Type 1 diabetes",
author = "DiLorenzo, {Teresa P.} and Lieberman, {Scott M.} and Toshiyuki Takaki and Shinichiro Honda and Chapman, {Harold D.} and Pere Santamaria and Serreze, {David V.} and Nathenson, {Stanley G.}",
note = "Funding Information: This work was supported by National Institutes of Health Grants AI07289 (S.G.N.), P01-DK52956 (S.G.N.), DK46266 (D.V.S.), DK51090 (D.V.S.), DK20541 (Albert Einstein College of Medicine Diabetes Research and Training Center), by grants from the Juvenile Diabetes Research Foundation International (to T.P.D., S.G.N., and D.V.S.), and by grants from the Natural Sciences and Engineering Council of Canada and the Canadian Institutes of Health Research (P.S.). The flow cytometry facility at Albert Einstein College of Medicine is supported by National Institutes of Health Cancer Center Grant CA13330. T.P.D. was a fellow of the Cancer Research Institute but is currently supported by a Career Development Award from the Juvenile Diabetes Research Foundation International, S.M.L. is supported by National Institutes of Health Medical Scientist Training Grant T32-GM07288, and P.S. is a Senior Scholar of the Alberta Heritage Foundation for Medical Research.",
year = "2002",
month = dec,
day = "1",
doi = "10.1006/clim.2002.5298",
language = "English (US)",
volume = "105",
pages = "332--341",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "3",
}