TY - JOUR
T1 - Double reactivity against actin and α-actinin defines a severe form of autoimmune hepatitis type 1
AU - Paul, Guéguen
AU - Georgios, Dalekos
AU - Jean-Baptiste, Nousbaum
AU - Kalliopi, Zachou
AU - Chaim, Putterman
AU - Pierre, Youinou
AU - Yves, Renaudineau
N1 - Funding Information:
Many thanks are due to Cindy Séné and Simone Forest for secretarial assistance. The authors are also grateful to Prof. Peter M. Lydyard (UCL Medical School, London, UK) for his thoughtful review of the manuscript. This work has been supported in part by a grant (#2466) of the Research Committee, University of Thessaly.
PY - 2006/11
Y1 - 2006/11
N2 - Anti-filamentous actin antibodies characterize autoimmune hepatitis type 1 (AIH-1). Recently, the binding domain of α-actinin on actin was shown to be a predominant epitope. To test this reactivity, an anti-α-actinin enzyme-linked immunosorbent assay was developed, and positivity confirmed by Western blot. Anti-α-actinin antibody was found in 21/50 (42%) of AIH-1 patients, compared with 52/401 (12.9%) of liver disease control patients, and with 6/200 (6%) of blood donors. Anti-filamentous and anti-α-actinin activities were found specifically together in 66% of anti-filamentous-positive AIH-1 patients. This combination of specificities reflected clinical and histological disease activity, short duration and absence of treatment. Finally, using an actin-α-actinin complex assay, the binding of anti-filamentous actin to α-actinin-binding domain on actin was demonstrated, as well as that of anti-α-actinin on the actin-binding domain of α-actinin. Thus, the frequent combination of anti-filamentous and anti-α-actinin antibodies seems to be the hallmark of activity in AIH-1.
AB - Anti-filamentous actin antibodies characterize autoimmune hepatitis type 1 (AIH-1). Recently, the binding domain of α-actinin on actin was shown to be a predominant epitope. To test this reactivity, an anti-α-actinin enzyme-linked immunosorbent assay was developed, and positivity confirmed by Western blot. Anti-α-actinin antibody was found in 21/50 (42%) of AIH-1 patients, compared with 52/401 (12.9%) of liver disease control patients, and with 6/200 (6%) of blood donors. Anti-filamentous and anti-α-actinin activities were found specifically together in 66% of anti-filamentous-positive AIH-1 patients. This combination of specificities reflected clinical and histological disease activity, short duration and absence of treatment. Finally, using an actin-α-actinin complex assay, the binding of anti-filamentous actin to α-actinin-binding domain on actin was demonstrated, as well as that of anti-α-actinin on the actin-binding domain of α-actinin. Thus, the frequent combination of anti-filamentous and anti-α-actinin antibodies seems to be the hallmark of activity in AIH-1.
KW - Active disease
KW - Anti-smooth-muscle antibodies
KW - Anti-ssDNA
KW - Autoimmunity
KW - Binding domain
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U2 - 10.1007/s10875-006-9045-z
DO - 10.1007/s10875-006-9045-z
M3 - Article
C2 - 17001515
AN - SCOPUS:33751120137
SN - 0271-9142
VL - 26
SP - 495
EP - 505
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
IS - 6
ER -