Dopamine receptors on human T- and B-lymphocytes

Laura Santambrogio; Maria Lipartiti; Alessandro Bruni; Roberto Dal Toso

doi:10.1016/0165-5728(93)90170-4

Dopamine receptors on human T- and B-lymphocytes

Laura Santambrogio, Maria Lipartiti, Alessandro Bruni, Roberto Dal Toso

Pathology

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

The presence of functional dopamine receptors on differentiated cells of the mammalian immune system is still under discussion. This study has utilized (-)-[³H]sulpride as a ligand to detect the presence of recognition sites of the dopamine D₂ receptor family on human T- and B-lymphocytes. The (-)-[³H]sulpiride binding was of high affinity (K_d 0.9 nM ± 0.2 nM, specific, saturable (B_max 10.2 ± 1.4 fmol/10⁶ cells) and reversible. The pharmacological characterization of the recognition site suggests, similarities mainly with the D₂ and D₄ rather than D₃ subtype of dopamine receptor. Furthermore, dopamine treatment was able to reduce the intracellular cAMP levels of lymphocytes stimulated with forskolin, thus suggesting a potential functional significance of this dopamine receptor in mediating neural-immune interactions.

Original language	English (US)
Pages (from-to)	113-119
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	45
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-5728(93)90170-4
State	Published - Jun 1993

Keywords

Dopamine receptor
Human lymphocyte
Neuroimmunomodulation
Sulpiride
cAMP

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/0165-5728(93)90170-4

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title = "Dopamine receptors on human T- and B-lymphocytes",

abstract = "The presence of functional dopamine receptors on differentiated cells of the mammalian immune system is still under discussion. This study has utilized (-)-[3H]sulpride as a ligand to detect the presence of recognition sites of the dopamine D2 receptor family on human T- and B-lymphocytes. The (-)-[3H]sulpiride binding was of high affinity (Kd 0.9 nM ± 0.2 nM, specific, saturable (Bmax 10.2 ± 1.4 fmol/106 cells) and reversible. The pharmacological characterization of the recognition site suggests, similarities mainly with the D2 and D4 rather than D3 subtype of dopamine receptor. Furthermore, dopamine treatment was able to reduce the intracellular cAMP levels of lymphocytes stimulated with forskolin, thus suggesting a potential functional significance of this dopamine receptor in mediating neural-immune interactions.",

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AU - Santambrogio, Laura

AU - Lipartiti, Maria

AU - Bruni, Alessandro

AU - Toso, Roberto Dal

PY - 1993/6

Y1 - 1993/6

N2 - The presence of functional dopamine receptors on differentiated cells of the mammalian immune system is still under discussion. This study has utilized (-)-[3H]sulpride as a ligand to detect the presence of recognition sites of the dopamine D2 receptor family on human T- and B-lymphocytes. The (-)-[3H]sulpiride binding was of high affinity (Kd 0.9 nM ± 0.2 nM, specific, saturable (Bmax 10.2 ± 1.4 fmol/106 cells) and reversible. The pharmacological characterization of the recognition site suggests, similarities mainly with the D2 and D4 rather than D3 subtype of dopamine receptor. Furthermore, dopamine treatment was able to reduce the intracellular cAMP levels of lymphocytes stimulated with forskolin, thus suggesting a potential functional significance of this dopamine receptor in mediating neural-immune interactions.

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KW - Sulpiride

KW - cAMP

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Dopamine receptors on human T- and B-lymphocytes

Abstract

Keywords

ASJC Scopus subject areas

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