DNase I hypersensitivity in the γ globin gene locus of K562 cells

Herbert M. Lachman, J. Gregory Mears

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We have probed the chromatin conformation of the GγA-γ-§β globin gene locus of K562 cells, a human hematopoietic cell line, with the enzyme pancreatic DNAse I. This enzyme preferentially digests genes in an active configuration. We have found that in K562 cells, which produce embryonic and fetal but not adult hemoglobins, both the active γ and inactive β genes are DNAse I sensitive. However, only the active γ genes have DNAse I hypersensitive regions. The hypersensitive regions have been mapped to an area approximately løø base pairs 5′ to the Gγ and Aγ genes.

Original languageEnglish (US)
Pages (from-to)6065-6077
Number of pages13
JournalNucleic acids research
Issue number17
StatePublished - Sep 10 1983

ASJC Scopus subject areas

  • Genetics


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