Abstract
Human and murine natural T (NT) cells, also referred to as NK1.1+ or NK T cells, express TCR with homologous V regions (hAV24/BV11 and mAV14/BV8, respectively) and conserved 'invariant' TCR AVAJ junctional sequences, suggesting recognition of closely related antigens. Murine NT cells recognize CD1-expressing cells and are activated in a CD1-restricted fashion by several synthetic α-glycosylceramides, such as α-GalCer. Here we studied the reactivity of human T cells against CD1d+ cells pulsed or not with α-GalCer and other related ceramides. CD1d-restricted recognition of α-GalCer was a general and specific feature of T cell clones expressing both BV11 and canonical AV24AJ18 TCR chains. Besides, human and murine NT cells showed the same reactivity patterns against a set of related glycosylceramides, suggesting a highly conserved mode of recognition of these antigens in humans and rodents. We also identified several AV24BV11 T cell clones self reactive against CD1+ cells of both hemopoietic and nonhemopoietic origin, suggesting the existence of distinct NT cell subsets differing by their ability to recognize self CD1d molecules.
Original language | English (US) |
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Pages (from-to) | 4391-4397 |
Number of pages | 7 |
Journal | European Journal of Immunology |
Volume | 28 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1998 |
Externally published | Yes |
Keywords
- CD1
- Glycosylceramide
- NKT cell
- TCR
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology